Abstract:
BACKGROUND:The lymphocyte transformation test (LTT) is used for in vitro diagnosis of drug hypersensitivity reactions. While its specificity is over 90%, sensitivity is limited and depends on the type of reaction, drug and possibly time interval between the event and analysis. Removal of regulatory T cells (Treg/CD25(hi)) from in vitro stimulated cell cultures was previously reported to be a promising method to increase the sensitivity of proliferation tests. OBJECTIVE:The aim of this investigation is to evaluate the effect of removal of regulatory T cells on the sensitivity of the LTT. METHODS:Patients with well-documented drug hypersensitivity were recruited. Peripheral blood mononuclear cells, isolated CD3(+) and CD3(+) T cells depleted of the CD25(hi) fraction were used as effector cells in the LTT. Irrelevant drugs were also included to determine specificity. (3)H-thymidine incorporation was utilized as the detection system and results were expressed as a stimulation index (SI). RESULTS:SIs of 7/11 LTTs were reduced after a mean time interval of 10.5 months (LTT 1 vs. LTT 2). Removal of the CD25(hi) fraction, which was FOXP3(+) and had a suppressive effect on drug-induced proliferation, resulted in an increased response to the relevant drugs. Sensitivity was increased from 25 to 82.35% with dramatically enhanced SI (2.05 to 6.02). Specificity was not affected. CONCLUSION:Removal of Treg/CD25(hi) cells can increase the frequency and strengths of drug-specific proliferation without affecting specificity. This approach might be useful in certain drug hypersensitivity reactions with borderline responses or long time interval since the hypersensitivity reaction.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Srinoulprasert Y,Pichler WJdoi
10.1159/000358491subject
Has Abstractpub_date
2014-01-01 00:00:00pages
198-205issue
3eissn
1018-2438issn
1423-0097pii
000358491journal_volume
163pub_type
杂志文章abstract::The relationship between the IgE load on mast cells and their secretory capacity when challenged with anti-IgE was studied in peritoneal cells obtained from rats of three different strains, Hooded Lister (HL), Wistar Kyoto (WKY) and Sprague-Dawley (SD). IgE was determined cytofluorometrically after labelling with FITC...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236849
更新日期:1995-03-01 00:00:00
abstract:AIM:We evaluated the effect of the 45-kD protein of Trichinella spiralis (gp45), purified by affinity chromatography, on random migration and chemotaxis, the oxidative metabolism of human neutrophils and on the CD11b upregulation induced by formyl-methionyl-leucyl-phenylalanine (f-MLP). METHODS:Donor neutrophils incub...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024359
更新日期:2000-05-01 00:00:00
abstract:BACKGROUND:The association between staphylococcal enterotoxins and atopic dermatitis (AD) is well characterized. We aim to evaluate the association between sensitization to staphylococcal enterotoxin A (SEA) and/or B (SEB) and the development of allergic airway disease. METHODS:Two hundred and seventy-four patients we...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000088868
更新日期:2005-12-01 00:00:00
abstract::Interaction between vascular cell adhesion molecule-1 (VCAM-1) on endothelial cells and CD49d molecules on eosinophils is thought to mediate the selective infiltration of eosinophils into inflamed tissues in allergic disease. IL-4 and TNF-alpha are associated with allergic conditions, and they have been shown to selec...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237357
更新日期:1996-10-01 00:00:00
abstract::A case of anaphylaxis following a bite from a Siberian hamster (SH; Phodopus sungorus) is described. Skin prick tests with hair, urine and salivary gland extracts from SH were positive, while the tests were negative for hair extracts from other rodents. IgE immunoblotting with the patient serum revealed 3 IgE-binding ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000324956
更新日期:2012-01-01 00:00:00
abstract::The present study was designed to determine if depletion of the complement system in the circulation with cobra venom factor (CVF) prevented the cellular infiltration in a guinea pig model of asthma. Guinea pigs were sensitized with ovalbumin (OA) alone or OA with complete Freund's adjuvant. Animals were pretreated wi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237214
更新日期:1996-02-01 00:00:00
abstract:BACKGROUND:Patients with chronic urticaria (CU) in the UK could be referred to health care professionals (HCPs) with diverse specialties using different guidelines. The aims of the present study were to determine which CU guidelines HCPs in the UK use, which tests they use for the diagnosis of CU, and how they manage C...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000430442
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Cow's milk (CM) allergy is the second most common food allergy developed during infancy in Japan. To identify predictors of persistent CM allergy, we investigated the tolerance acquisition rate based on an oral food challenge in children under 6 years of age, diagnosed with immediate-type CM allergy. METHOD...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000486311
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Sweating can worsen atopic dermatitis (AD). The purpose of this work was to study the associations between reactivity to autologous sweat and the clinical severity of AD as well as investigate the possible wheal-inducing factors of sweat. METHODS:Intracutaneous skin tests with autologous sweat were performe...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000447638
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:IgE mediates immediate-type hypersensitivity reactions responsible for various allergic symptoms. It is secreted by IgE-producing plasma cells, which differentiate from B cells expressing membrane-bound IgE (mIgE) on their surface. The epsilon-chain of human mIgE contains a membrane-anchoring peptide and an ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000063860
更新日期:2002-08-01 00:00:00
abstract:BACKGROUND:Clinically it is recognized that tree nut allergies such as hazelnut allergy are not usually outgrown. Specific mechanisms underlying the persistence of such food allergies are incompletely understood. Here we studied the natural history and the long-term immune and clinical characteristics of hazelnut aller...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000283028
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:Allergic bronchopulmonary aspergillosis (ABPA) develops in the presence of predisposing conditions such as asthma and cystic fibrosis. Even ABPA accompanied by asthma is often complicated by chronic Pseudomonas aeruginosa or nontuberculous mycobacterial infection of the lower respiratory tract, rendering tre...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000507216
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Japanese cedar pollinosis (JCPsis) is an immunoglobulin E-mediated type I allergy caused by exposure to Japanese cedar pollen (JCP). Blood thymus and activation-regulated chemokine (TARC) levels are well known as an objective parameter for disease severity for several allergic disorders. The present study ai...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000103223
更新日期:2007-01-01 00:00:00
abstract::A prospective study was conducted on 73 allergic patients and 14 normal volunteers in order to measure the IgE concentration in both serum and saliva, and analyze salivary IgE according to the characteristics of the allergic disease. IgE was more frequently detected in saliva from allergic patients than from normal su...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236715
更新日期:1994-05-01 00:00:00
abstract:BACKGROUND:Nasal polyps are a common health problem that can significantly impact the quality of life. OBJECTIVE:To analyze the impact of allergy and peripheral eosinophils (EOS) on the morbidity of chronic rhinosinusitis with nasal polyps (CRSwNP) in Northwest China. METHODS:A retrospective cohort of 323 patients wh...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000497384
更新日期:2019-01-01 00:00:00
abstract::Hypersensitivity pneumonitis remains an important industrial disease in mushroom workers. It has a significant morbidity, and early diagnosis and removal from exposure to the antigen are critically important in its management. Recently, several new allergens have been described, particularly those from mushroom specie...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000083298
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:Common variable immunodeficiency (CVID) is the most common primary antibody deficiency syndrome in humans, but it remains a diagnosis of exclusion in most cases. Several genetically defined primary immunodeficiencies mimic CVID. Among them is the X-linked lymphoproliferative syndrome (XLP) which was shown to...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000135702
更新日期:2008-01-01 00:00:00
abstract::Aims of the Monaco Charter: (1) to present the current evidence on the efficacy and safety of allergen-specific immunotherapy (SIT) and to address the reasons for its underuse in clinical practice; (2) to develop strategies to increase the awareness about the benefits and the hazards of SIT in allergic patients, lay p...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000343883
更新日期:2013-01-01 00:00:00
abstract::Microwave radiation from mobile phones enhanced skin wheal responses induced by house dust mite and Japanese cedar pollen while it had no effect on wheal responses induced by histamine in patients with atopic eczema/dermatitis syndrome (AEDS). Microwave radiation also increased plasma levels of substance P (SP) and va...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000067592
更新日期:2002-12-01 00:00:00
abstract::The modulatory role of hormones in the regulation of the immune response has been well documented. Here, we present some thoughts on how sex hormones affect immunity, particularly at mucosal sites. We further discuss the possible pitfalls and difficulties associated with analysis of the data. ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000236631
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:A number of different molecules are known to be involved in the signal pathway to release histamine from mast cells, among which arachidonic acid (AA) is one of the key mediators. On the other hand, we found that the application of compound 48/80, a typical histamine liberator, generated superoxide in mast c...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000070216
更新日期:2003-04-01 00:00:00
abstract:BACKGROUND:Previous studies suggest that administration of probiotics in vitro can stimulate regulatory and Th1 immune responses. We studied both the in vitro immunological effects of probiotics and the ex vivo immunological effects after oral administration of probiotics in children with food allergy, a Th2-mediated d...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000099467
更新日期:2007-01-01 00:00:00
abstract::Aerosolized monoclonal antibodies (mAbs) specific for T-cell receptors (TCR) were used to manipulate T-cell function in airways of ovalbumin (OVA)-sensitized and -challenged mice with airway hyperresponsiveness (AHR). The inhaled mAbs were found to be effective at low doses, had little or no systemic effect and specif...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000077533
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND:Interleukin (IL)-33, a new member of the IL-1 cytokine family, has been recognized as a key cytokine that enhances T helper 2-balanced immune regulation through its receptor ST2; however, the function and relationship of the IL-33 and ST2 pathways in bronchial asthma are still unclear. We investigated the ce...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000327259
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Exposure and sensitization to indoor allergens have been associated with the development of asthma and other allergic diseases in many parts of the world. It is important to establish the degree of exposure, and to evaluate whether allergen control measures are effective in a particular area. METHODS:Concen...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000077356
更新日期:2004-04-01 00:00:00
abstract:INTRODUCTION:Obesity-associated asthma is characterized by type 2-low airway inflammation. We previously showed that EM900, which is a 12-membered nonantibiotic macrolide, suppressed airway inflammation in a mouse model of asthma exacerbation. The aim of this study was to clarify the effects of EM900 in obesity-associa...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000508709
更新日期:2020-01-01 00:00:00
abstract::Monocytes and lymphocytes from human blood contain 0.043 +/- 0.007 and 0.053 +/- 0.014 pg histamine/cell, respectively, which can be released by a number of stimulants (A 23187, C5a, substance P, specific allergen). The release process takes 60-120 min to reach its end point, in contrast to tissue mast cells which com...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236974
更新日期:1995-05-01 00:00:00
abstract:BACKGROUND:The production of house dust mite (HDM) allergen products for allergy immunotherapy has traditionally been based on purified mite bodies or whole-mite culture, which are quite different source materials with a limited possibility for adjusting the chemical composition. The SQ HDM SLIT-tablet is a fast-dissol...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000444016
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Airway closure is frequently observed in human asthma. However, limited information exists on the factors that cause this condition. In this study, an allergic cynomolgus monkey model was used to characterize the condition of airway closure and assess the contribution of histamine H1 receptors to this respon...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000084611
更新日期:2005-05-01 00:00:00
abstract::Reversible airway obstruction and airway inflammation following allergen exposure represent crucial pathophysiological features of allergic asthma. In order to investigate these events, bronchial-specific challenge is commonly employed although it may be complicated and may not be free of possible risks. Therefore, we...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237461
更新日期:1997-03-01 00:00:00