Abstract:
BACKGROUND:Previous studies suggest that administration of probiotics in vitro can stimulate regulatory and Th1 immune responses. We studied both the in vitro immunological effects of probiotics and the ex vivo immunological effects after oral administration of probiotics in children with food allergy, a Th2-mediated disease. METHODS:Thirteen children were enrolled. Probiotics (n = 7) or placebo (n = 6) were orally administered during 3 months. At baseline and after 1 and 3 months, peripheral blood mononuclear cells were stimulated with crude peanut extract, anti-CD3, or anti-CD40 and IL-4 in the presence (in vitro response) or absence (ex vivo response) of probiotics. The proliferation and production of IFN-gamma, IL-5, IL-13, IL-10, TNF-alpha, IL-6 and IgE were analyzed. Sensitization to peanut, cow's milk and hen's egg was determined before and after treatment. RESULTS:The in vitro addition of probiotics to peripheral blood mononuclear cell cultures resulted in enhanced proliferation and production of IFN-gamma, IL-10 and TNF-alpha. After oral treatment, proliferation in the presence of probiotics increased, whereas in vitro IgE production decreased in the probiotics group compared to baseline. The ex vivo production of IL-10, TNF-alpha and IL-6 tended to decrease. Th1 and Th2 cytokines were not altered. Sensitization remained unchanged. CONCLUSION:Probiotics enhanced the production of Th1 and regulatory cytokines in vitro. Oral administration of probiotics resulted in a slightly decreased ex vivo production of IL-10, TNF-alpha and IL-6. This indicates that probiotics have a different potential to modulate the immune response in vitro versus ex vivo.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Flinterman AE,Knol EF,van Ieperen-van Dijk AG,Timmerman HM,Knulst AC,Bruijnzeel-Koomen CA,Pasmans SG,van Hoffen Edoi
10.1159/000099467subject
Has Abstractpub_date
2007-01-01 00:00:00pages
237-44issue
3eissn
1018-2438issn
1423-0097pii
000099467journal_volume
143pub_type
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
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abstract::Profilins are ubiquitous proteins, present in all eukaryotic cells and identified as allergens in pollen, latex and plant foods. The highly conserved structure justifies the cross-reactive nature of IgE antibodies against plant profilins and their designation as pan-allergens. Primary sensitization to profilin seems t...
journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236767
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journal_title:International archives of allergy and immunology
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journal_title:International archives of allergy and immunology
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pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
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更新日期:1996-03-01 00:00:00
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更新日期:2016-01-01 00:00:00
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053600
更新日期:1999-01-01 00:00:00
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journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000508522
更新日期:2020-01-01 00:00:00