Abstract:
AIM:We evaluated the effect of the 45-kD protein of Trichinella spiralis (gp45), purified by affinity chromatography, on random migration and chemotaxis, the oxidative metabolism of human neutrophils and on the CD11b upregulation induced by formyl-methionyl-leucyl-phenylalanine (f-MLP). METHODS:Donor neutrophils incubated with different amounts of gp45 (0.5, 1, 1.5, 2 microg/ml) or buffer and the random migration and chemotaxis, evaluated by means of a special technique of image analysis, and the chemiluminescence response to f-MLP or phorbol myristate acetate (PMA) were analyzed. The effect on CD11b upregulation was assessed incubating cells with the protein, when activating them with f-MLP. RESULTS:The results showed that gp45 inhibited both random and stimulated migrations, and reduced the response to f-MLP and PMA. Furthermore, gp45 significantly reduced the upregulation of the CD11b induced by f-MLP. CONCLUSION:The results show that gp45 inhibits PMN in different functions, suggesting an anti-inflammatory action.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Bruschi F,Carulli G,Azzarà A,Homan W,Minnucci S,Rizzuti-Gullaci A,Sbrana S,Angiolini Cdoi
10.1159/000024359keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
58-65issue
1eissn
1018-2438issn
1423-0097pii
24359journal_volume
122pub_type
杂志文章abstract:BACKGROUND:Pseudomonas aeruginosa phospholipase C (PLC) is a critical component in the pathogenesis of severe P. aeruginosa infections. However, P. aeruginosa can produce a hemolytic (PLC-H) as well as a nonhemolytic (PLC-N) variant, both having a MW of about 77 kD. In the past, studies did not distinguish between both...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237441
更新日期:1997-02-01 00:00:00
abstract:BACKGROUND:The aim was to determine the clinical characteristics of patients with seasonal rhinitis (SR) and to disclose differences in the treatment of SR between an adult allergy clinic and other clinics over time. METHODS:A retrospective study was conducted based on clinical records of 774 out of 955 patients diagn...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000087360
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND:The aetiological factors underlying the worldwide increase in the prevalence of asthma and the international patterns of the prevalence of asthma are not well understood. This has led to consideration of factors such as fetomaternal health which may programme the initial susceptibility to allergic sensitisat...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024149
更新日期:1999-02-01 00:00:00
abstract:BACKGROUND:Farming has been widely reported to be associated with decreased risk of developing atopic disorders, but underlying immunomodulatory mechanisms are still not fully defined. We delineated T-cell functions after induction of mucosal tolerance in the context of intranasally delivered organic dust compounds, li...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000128583
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Disturbed cytokine production is thought to govern inflammation in asthma, which, in its turn, may lead to uncontrolled disease. The aim of this study was to assess the relationship between cytokine production from blood leucocytes and the level of asthma control. METHODS:We compared the production of inter...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000329858
更新日期:2012-01-01 00:00:00
abstract::We have systematically investigated the capacity of highly purified human peripheral CD8+ T cells to produce interleukin (IL)-4 and interferon (IFN)-gamma when triggered by different stimuli. CD8+ T cells were isolated from peripheral blood by positive selection to > 99% purity and stimulated with one of three differe...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237030
更新日期:1995-05-01 00:00:00
abstract:BACKGROUND:Food allergy to wheat-derived foodstuffs is on the rise. Tri a 14, a wheat flour lipid transfer protein (LTP) allergen, has been described as a major allergen associated with baker's asthma and wheat food allergy. Cross-reactivity among LTP allergens leads to the so-called 'LTP syndrome'. METHODS:Eight adul...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000265539
更新日期:2010-01-01 00:00:00
abstract::Peritoneal mast cells of Syrian hamsters release histamine to the action of concanavalin A (Con A) in dose-dependent fashion. The rate of release was very rapid in the first seconds of cell activation and completed in 60 s after the challenge. Morphological changes concomitant to the lectin treatment, followed by elec...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236113
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:The allergenic dust mite species Dermatophagoides pteronyssinus and Dermatophagoides farinae generally inhabit warm moist environments. This study tested the hypothesis that these allergenic species may thrive in air conditioner filters. METHODS:A year-long investigation of the dust mite population densitie...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000102619
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Chronic rhinosinusitis (CRS) is a multifaceted disease with a significant genetic component. The importance of taste receptor signaling has recently been highlighted in CRS; single nucleotide polymorphisms (SNPs) of bitter tastant-responsive G-protein-coupled receptors have been linked with CRS and with alte...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000499875
更新日期:2019-01-01 00:00:00
abstract:INTRODUCTION:Unique gut microbial colonisation patterns are associated with the onset of allergic disease in infants; however, there is insufficient evidence to determine if aberrant microbial composition patterns persist in adult allergic rhinitis (AR) sufferers. OBJECTIVE:To compare the gut microbiome composition be...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000510536
更新日期:2021-01-01 00:00:00
abstract::Sera of 54 symptomatic workers showing sensitization to isocyanate-human serum albumin (HSA) conjugates were subjected to parallel enzyme allergosorbent test (EAST) and CAP measurements to determine IgE antibodies to diphenyl-methane diisocyanate-HSA, toluene diisocyanate-HSA and hexamethylene diisocyanate-HSA. Result...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237325
更新日期:1996-08-01 00:00:00
abstract::The morbidity and mortality of elderly onset asthma have recently been on the increase. To evaluate the allergic status of the elderly onset asthmatics, we performed skin tests for allergens and estimated serum total IgE levels of the patients. The proportion of the skin-test-positive patients among the elderly onset ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053600
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:To elucidate the usefulness of antigen-specific immunotherapy based on oral vaccination with an edible part of the plant, we examined the effect of transgenic (Tg) rice seeds expressing an immunodominant fragment of the group 1 antigen of Dermatophagoides pteronyssinus (Der p 1) on a murine model of asthma. ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000210649
更新日期:2009-01-01 00:00:00
abstract::The clear recognition that asthma has an inflammatory basis has led to a search for genetic and environmental causes. Genetic linkage studies provide evidence for allelic association between polymorphic markers of interleukin-9 on chromosome 5 and a principal component of the allergic phenotype, log IgE. Further studi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000236921
更新日期:1995-05-01 00:00:00
abstract:BACKGROUND:Dietary intake of wheat causes hypersensitivity reactions in patients suffering from IgE-mediated food allergy and coeliac disease. AIM:To study the expression of IgE- and IgA-reactive antigens during wheat seed maturation and in different wheat cultivars. METHODS:Summer wheat was grown in a glasshouse and...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000199713
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Chronic urticaria is associated with an immune dysregulation usually mediated by T lymphocytes. Recently, Th17 and Tc17 have been implicated in autoimmune diseases; however, their role in urticaria is not clear yet. METHODS:For the study we recruited 20 patients [10 of them had autoreactive chronic spontane...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000345401
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Cross-sectional epidemiological studies have demonstrated that farm milk from traditional farm settings possesses allergoprotective properties. Up to now, it has not been clarified which milk ingredient is responsible for protection against allergic diseases. As farm milk is rich in conjugated linoleic acids...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000358523
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Transforming growth factor-β1 (TGF-β1)-in-duced proliferation of airway smooth muscle cells plays critical roles in the development of airway remodeling. Six1 (sine oculis homeobox homolog 1) has been demonstrated to be involved in airway inflammation and remodeling in asthmatic mice. OBJECTIVES:The aim of ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000505064
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Penicillium chrysogenum (Penicillium notatum) is a prevalent airborne Penicillium species. A 34-kD major IgE-reacting component from P. chrysogenum has been identified as an alkaline serine protease (Pen ch 13, also known as Pen n 13 before) by immunoblot and N-terminal amino acid sequence analysis. METHODS...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000048165
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:Ultra-rush regimens for administering sublingual immunotherapy to patients with allergies are becoming more widespread. We aimed to assess treatment safety for patients with allergic rhinitis with or without asthma caused by Dermatophagoides house dust mites. METHODS:This observational study at 5 Spanish ce...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1159/000319211
更新日期:2011-01-01 00:00:00
abstract::We describe an HLA-B8, DR3-positive patient with localized morphea after silicone gel breast implantation for cosmetic reasons. We believe that this case suggests that a genetic background, i.e. HLA-B8, DR3 haplotype, is involved in the autoimmune response to silicone. ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237437
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Patients with atopic dermatitis (AD) are frequently colonized with α-toxin-producing Staphylococcus aureus which is in turn positively correlated with the severity of eczema. METHODS:IN this study we addressed T cell proliferation and T cell as well as monocyte cytokine secretion upon α-toxin stimulation in...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000323905
更新日期:2011-01-01 00:00:00
abstract::Increased serum IgE and enhanced susceptibility to viral infections, decreased levels of interferons, lymphocytic skin infiltrates and IgE-bearing epidermal Langerhans cells are striking features in patients with atopic eczema (AE). Since the hyper-IgE syndrome is known to improve under alpha-interferon (alpha-IFN) th...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236494
更新日期:1993-01-01 00:00:00
abstract::In this study, we postulated that repeated cycles of IgE passive sensitisation and antigen challenge may play a role in up-regulating eosinophil response in allergic conditions. Antigen-mediated stimulation of the pleural cavity of rats passively sensitised with a single injection of IgE anti-DNP resulted in mast cell...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000049530
更新日期:2001-12-01 00:00:00
abstract:BACKGROUND:Although common variable immunodeficiency (CVID) is associated with sinopulmonary disease, there are no previous systematic evaluations comparing computed tomographic (CT) sinus imaging with immunoglobulin measurements in patients with this condition. METHODS:We performed a retrospective review by a blinded...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000260088
更新日期:2010-01-01 00:00:00
abstract::Mast cell hyperplasia is found in different pathologies such as chronic inflammatory processes, fibrotic disorders, wound healing or neoplastic tissue transformation. The functional significance of the accumulation of mast cells in these processes is largely unknown. It is now established that bone marrow-derived mast...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000048181
更新日期:2002-02-01 00:00:00
abstract::Superoxide (O2-) and nitric oxide (NO) production by polymorphonuclear leukocyte (PMNs) and monocytes in patients with liver cirrhosis were evaluated. PMNs obtained from cirrhotic patients were less effective than those from controls in producing O2- after stimulation with opsonized zymosan, while they were more effec...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236975
更新日期:1995-05-01 00:00:00
abstract:BACKGROUND:Protease-activated receptors (PARs) are a unique class of G-protein-coupled receptors, which are activated by proteolytic cleavage of the amino terminus of the receptor itself. Although expression of the PAR1, which is typically activated by thrombin, on human eosinophils has been demonstrated, no effect of ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000080037
更新日期:2004-09-01 00:00:00
abstract::Various methods have been devised to assay eosinophil chemotaxis. In this review, we introduced a rapid and sensitive eosinophil chemotactic assay system based on eosinophil peroxidase (EPO) determination. Eosinophils were semipurified by dextran sedimentation followed by Percoll gradient centrifugation. Eosinophil mi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053624
更新日期:2000-05-01 00:00:00