Inhibitory effects of human neutrophil functions by the 45-kD glycoprotein derived from the parasitic nematode Trichinella spiralis.

Abstract:

AIM:We evaluated the effect of the 45-kD protein of Trichinella spiralis (gp45), purified by affinity chromatography, on random migration and chemotaxis, the oxidative metabolism of human neutrophils and on the CD11b upregulation induced by formyl-methionyl-leucyl-phenylalanine (f-MLP). METHODS:Donor neutrophils incubated with different amounts of gp45 (0.5, 1, 1.5, 2 microg/ml) or buffer and the random migration and chemotaxis, evaluated by means of a special technique of image analysis, and the chemiluminescence response to f-MLP or phorbol myristate acetate (PMA) were analyzed. The effect on CD11b upregulation was assessed incubating cells with the protein, when activating them with f-MLP. RESULTS:The results showed that gp45 inhibited both random and stimulated migrations, and reduced the response to f-MLP and PMA. Furthermore, gp45 significantly reduced the upregulation of the CD11b induced by f-MLP. CONCLUSION:The results show that gp45 inhibits PMN in different functions, suggesting an anti-inflammatory action.

authors

Bruschi F,Carulli G,Azzarà A,Homan W,Minnucci S,Rizzuti-Gullaci A,Sbrana S,Angiolini C

doi

10.1159/000024359

keywords:

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

58-65

issue

1

eissn

1018-2438

issn

1423-0097

pii

24359

journal_volume

122

pub_type

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