Abstract:
BACKGROUND:Protease-activated receptors (PARs) are a unique class of G-protein-coupled receptors, which are activated by proteolytic cleavage of the amino terminus of the receptor itself. Although expression of the PAR1, which is typically activated by thrombin, on human eosinophils has been demonstrated, no effect of thrombin on eosinophil function has been shown yet. Thus we investigated whether thrombin affects eosinophil migration in vitro. METHODS:Eosinophils were obtained from venous blood of healthy donors. Cell migration was studied by micropore filter assays. Involvement of PARs in thrombin-dependent migration was tested functionally using selective agonist peptides for PARs and a cleavage blocking PAR1 antibody. RESULTS:Thrombin significantly stimulated eosinophil chemotaxis in a dose-dependent manner. This effect was mimicked by the PAR1 but not the PAR2 agonist and was reversed by the cleavage blocking PAR1 antibody. Checkerboard experiments indicated that eosinophil migration depends on the presence of thrombin in a concentration gradient. CONCLUSIONS:Data suggest that activation of PAR1 by thrombin stimulates directed migration of human eosinophils and thereby may affect eosinophils in tissue and allergic inflammation.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Feistritzer C,Mosheimer BA,Kaneider NC,Riewald M,Patsch JR,Wiedermann CJdoi
10.1159/000080037keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
12-6issue
1eissn
1018-2438issn
1423-0097pii
80037journal_volume
135pub_type
杂志文章abstract:BACKGROUND:Cannabinoid (CB) 2 is expressed on immune and inflammatory cells. Identification of 2-arachidonyl glycerol (2-AG) and anandamide as endogenous CB2 ligands has allowed investigations of the roles of CB2 and its endogenous ligand system in inflammatory cells. However, the roles of this receptor-ligand system i...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000336167
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:There seems to be an association between paracetamol consumption during late pregnancy and the prevalence of wheezing in infancy and childhood. The aim of the present study is to determine whether the aforementioned association is modified by the presence of asthma in the mother. METHODS:A total of 1,741 ch...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000176304
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Wheat is known as the most widely consumed food all over the world. Although many types of wheat allergy have been recognized, their treatment still has a long way to go due to the complex pathogenesis. Oral immunotherapy (OIT) is under investigation for the treatment of wheat allergies. Previous studies hav...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000506945
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Recently, the relationship between antigen contact via skin (skin sensitization) and the development of food allergies has gained increasing attention. However, few studies have examined the effects of skin sensitization on healthy skin. OBJECTIVE:To examine the effect of sensitization in healthy skin on Ig...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000511239
更新日期:2020-11-11 00:00:00
abstract:BACKGROUND:Cytosolic phospholipase A2 (cPLA2) plays a key role in the development of late-phase anaphylaxis. L-Glutamine (Gln), a nonessential amino acid, has anti-inflammatory activity via inhibiting cPLA2. METHODS:We used a penicillin-induced murine model of anaphylaxis, and late-phase anaphylaxis was quantified by ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000452103
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by the interplay between multiple genetic and environmental factors. The pathogenesis of AD remains incompletely understood. Treatment with topical steroids for chronic AD symptoms has severe side effects and so a new treatment is re...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000486958
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Evidence regarding sublingual immunotherapy (SLIT) efficacy and its good safety profile has been demonstrated with pollen and house dust mite (HDM) allergens in the treatment of airway allergies. In addition, the use of grass pollen presents a SLIT disease-modifying treatment for respiratory allergies. OBJE...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000446155
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Th17 is a subset of T-helper lymphocytes that produce proinflammatory cytokines, mainly IL-17. Serum IL-17 is increased in allergic patients and relates to clinical severity. Recently, it has been reported that CD161 is a highly upregulated gene in Th17 clones and all IL-17-producing cells are contained in C...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000330903
更新日期:2012-01-01 00:00:00
abstract::Platelet-activating factor (PAF)-induced augmentation of spontaneous differentiation into eosinophil-lineage cells from hematopoietic precursor cells was found among human umbilical cord blood mononuclear cells (CBMCs). This ability of PAF to augment eosinophil production was significantly (p < 0.01) inhibited by the ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236586
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Hymenoptera venom allergy (HVA) has a prevalence of 3% in adults. Although patients have no ongoing symptoms, they often suffer from an impairment of their psychological well-being and quality of life. OBJECTIVE:The aim of this study was to analyze sex-specific differences regarding the psychological burden...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000507625
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Sweating can worsen atopic dermatitis (AD). The purpose of this work was to study the associations between reactivity to autologous sweat and the clinical severity of AD as well as investigate the possible wheal-inducing factors of sweat. METHODS:Intracutaneous skin tests with autologous sweat were performe...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000447638
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Recombinant dog allergens, rCan f 1 and rCan f 2, and their antibodies are good tools for the characterization of dog allergens in order to develop modern therapeutic and preventive methods for dog allergy. METHODS:In this study, cDNA was synthesized from the mRNA of dog salivary glands and cloned into the ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000097498
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Egg allergy is one of the most common food allergies in children. The standard therapy for egg allergy is strict avoidance. Yet, there is considerable clinical and scientific interest in primary or secondary prevention. A major drawback of oral tolerance (OT) induction protocols, however, is the possibility ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000363110
更新日期:2014-01-01 00:00:00
abstract::Endogenous peroxidase has been reported in rat peritoneal mast cells and granules. Mast cell granules have also been shown to avidly bind exogenous eosinophil peroxidase. To examine the possibility that contaminating eosinophil peroxidase contributes to the reported rat mast cell peroxidase activity, mast cells were i...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236655
更新日期:1994-01-01 00:00:00
abstract::HIV-1 RNA has become the standard for monitoring antiretroviral therapies. Dogma predicts, however, that a viral protein like p24 should be at least as good a marker of HIV disease activity, provided that it is measured with sufficient sensitivity and accuracy. Simple modifications including use of a more efficient vi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000074552
更新日期:2003-11-01 00:00:00
abstract:BACKGROUND:IgE-mediated hypersensitivity to latex has been recognized as an increasing health problem with far-reaching consequences for patients, regarding both their occupational situation and safety in medical care. Therefore, a correct diagnosis of natural rubber latex (NRL) allergy is essential. The purpose of the...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000049518
更新日期:2001-11-01 00:00:00
abstract::Cross-linking of FcepsilonRI on mast cells elicits positive signal transduction cascades that lead to the release of a variety of proinflammatory mediators. Mouse mast cells also express gp49B1 on their surface, an immunoglobulin superfamily member that bears two immunoreceptor tyrosine-based inhibitory motifs in its ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024059
更新日期:1999-02-01 00:00:00
abstract::Kimura disease is a rare disorder of unknown etiology, characterized by the presence of benign subcutaneous granuloma, marked peripheral blood eosinophilia and elevation of the immunglobulin E (IgE) serum level. Here, we present a case of a 12-year-old boy with Kimura disease who had a history of repeated severe influ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000337777
更新日期:2012-01-01 00:00:00
abstract::Ever since the realization that T lymphocytes are key players in the defense against pathogens and tumors, a major aim of immunologists has been to understand the relationship between the functional and migratory properties of antigen-specific T cells. The current paradigm proposes that T cells follow organ-specific t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000074896
更新日期:2003-12-01 00:00:00
abstract:BACKGROUND:omega-5 gliadin is a major allergen in exercise-induced wheat allergy (EIWA), but it is also implicated in immediate-type reactions to wheat. An ImmunoCAP assay to measure omega-5 gliadin-specific IgE has become available. This study aimed to evaluate this new biological test in wheat allergy diagnosis and t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000176309
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Recent studies have demonstrated that eosinophils from allergic patients express low levels of FcepsilonRI on their surface, but the regulatory mechanisms of eosinophil surface FcepsilonRI expression are not fully understood. We investigated whether IL-4 and IgE, which are reported to regulate surface Fcepsi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053782
更新日期:2001-04-01 00:00:00
abstract:BACKGROUND:Protective acquired immunity against helminths and allergic sensitisation are both characterised by high IgE antibody levels. Levels of IgE antibodies are naturally tightly regulated by several mechanisms including binding of the CD23 receptor. Following observations that helminth infections and allergic sen...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000346545
更新日期:2013-01-01 00:00:00
abstract::Hypersensitivity pneumonitis remains an important industrial disease in mushroom workers. It has a significant morbidity, and early diagnosis and removal from exposure to the antigen are critically important in its management. Recently, several new allergens have been described, particularly those from mushroom specie...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000083298
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:Adoptive transfer of helper T (Th) cells conferred a late asthmatic response upon antigen challenge. A possible production of a contractile activity for bronchial smooth muscle (BSM) by activated Th cells was examined. METHOD:Murine Th clones were stimulated with immobilized anti-CD3 antibody in the presenc...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000337743
更新日期:2012-01-01 00:00:00
abstract::We describe an HLA-B8, DR3-positive patient with localized morphea after silicone gel breast implantation for cosmetic reasons. We believe that this case suggests that a genetic background, i.e. HLA-B8, DR3 haplotype, is involved in the autoimmune response to silicone. ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237437
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Flavonoids exert antiallergic and antioxidant effects. We investigated the efficacy of enzymatically modified isoquercitrin (EMIQ), a flavonoid, to relieve symptoms of pollinosis. METHODS:In a parallel-group, double-blind placebo-controlled study design, 20 subjects with Japanese cedar pollinosis took two c...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000205582
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Common variable immunodeficiency (CVID) is characterized by hypogammaglobulinemia and a defect in antibody production. Herein we describe 3 patients diagnosed with CVID in whom vitamin D deficiency was detected in the absence of enteropathy. METHODS:Biochemical and immunological analysis, serum osteocalcin,...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000128661
更新日期:2008-01-01 00:00:00
abstract::Bronchial epithelial cells play an important role in the pathogenesis of some inflammatory diseases of bronchial mucosa. Epithelial-cell-derived cytokines are important in the elucidation of the mechanism by which airway inflammation occurs, especially in respiratory virus infection, because these cells are the primar...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237584
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND:The allergenic dust mite species Dermatophagoides pteronyssinus and Dermatophagoides farinae generally inhabit warm moist environments. This study tested the hypothesis that these allergenic species may thrive in air conditioner filters. METHODS:A year-long investigation of the dust mite population densitie...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000102619
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:The development of simple and standardised methods to measure airborne levels of workplace biological allergens is an important step in reducing the incidence of occupational asthma. Such a method would be useful for measuring wheat flour allergens which cause asthma in bakers. Measurement of allergen per se...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237680
更新日期:1997-11-01 00:00:00