Abstract:
BACKGROUND:Pseudomonas aeruginosa phospholipase C (PLC) is a critical component in the pathogenesis of severe P. aeruginosa infections. However, P. aeruginosa can produce a hemolytic (PLC-H) as well as a nonhemolytic (PLC-N) variant, both having a MW of about 77 kD. In the past, studies did not distinguish between both types of PLC with regard to the induction of inflammatory mediators from human cells. METHODS:We compared the ability of P. aeruginosa PLC-H and PLC-N to generate leukotriene B4 (by HPLC) and oxygen (O2-) metabolites (luminol-enhanced chemiluminescence), and to release beta-glucuronidase and histamine (fluorophotometry from human granulocytes. Therefore, human neutrophilic granulocytes (PMN; 1 x 10(7)) or human peripheral blood mononuclear cells (5 x 10(6)) were treated with purified P. aeruginosa PLC-H (up to 10 units) as well as culture supernatants (cutoff: MW > 50,000) of P. aeruginosa PAOl capable of producing both PLC-H and PLC-N, and PAOl mutant strains deficient in the production of either or both phospholipases. Controls were PLC-H from Clostridium perfringens and PLC-N from Bacillus cereus. RESULTS:PLC-H-containing P. aeruginosa culture supernatant, purified P. aeruginosa PLC-H as well as PLC-H from P. perfringens activated human leukocytes for a significant (p < 0.05) increase in inflammatory mediator release. In this regard, purified PLC-H (10 units) from P. aeruginosa activated human PMN for a significant increase in the generation of oxygen metabolites (30 +/- 5.4 x 10(3) cpm) and in leukotriene B4 (6.1 +/- 2.0 ng), in the release of beta-glucuronidase (15.8 +/- 1.1%) and of histamine (25.8 +/- 6.2%) as compared to the corresponding control values (3 +/- 1 x 10(3) cpm; 0.2 +/- 0.1 ng; 5.1 +/- 1.0%, 5.1 +/- 1.5%). Culture supernatants containing no PLC or only PLC-N, as well as PLC-N from B. cereus, failed to activate or only slightly stimulated human granulocytes for inflammatory mediator release. CONCLUSION:The data thus provide evidence that P. aeruginosa PLC-H can be a potent inducer of inflammatory mediator release, at least in vitro. Our results therefore contribute to the understanding of the pathophysiological role of P. aeruginosa PLCs.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
König B,Vasil ML,König Wdoi
10.1159/000237441subject
Has Abstractpub_date
1997-02-01 00:00:00pages
115-24issue
2eissn
1018-2438issn
1423-0097journal_volume
112pub_type
杂志文章abstract:BACKGROUND:The classical clinical manifestation of untreated immunoglobulin deficiency comprises predominantly recurrent and complicated respiratory tract infections. Before the 1980s, little was known about the clinical manifestation of immunodeficiency in the general medical population, and also the availability of s...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000301584
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:High antibiotic prescribing rates for adults with an asthma exacerbation have been reported in developed countries, but few studies have assessed the variation of antibiotic and adjunctive treatment in the routine care of children. OBJECTIVE:We evaluated the trends in health resource utilization for childre...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000507857
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Allergies and asthma exhibit a growing health problem in the world. Sparse data are available about the prevalence of allergic sensitization in Estonia and Eastern Europe as well. We studied the prevalence of allergic sensitization, the associations between respiratory symptoms and allergic sensitization, an...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000097027
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Shrimp sensitization is common in the general population, but the presence of symptoms is only moderately related to sensitization. A point still at issue is which in vivo and/or in vitro tests (food challenge, component-resolved diagnosis, house dust mite [HDM] sensitization) can help in distinguishing shri...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000481985
更新日期:2017-01-01 00:00:00
abstract::Allergens present in the excretory/secretory (ES) products of adult Paragonimus ohirai were biochemically identified. Immunoblot analysis using sera from P. ohirai-infected rats revealed only two allergens to be major proteins in the ES products, with apparent molecular masses (M(r)) of 27 and 29 kD. As the ES product...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000064251
更新日期:2002-07-01 00:00:00
abstract::A high-dose, accelerated escalation schedule during subcutaneous allergen-specific immunotherapy (AIT) is safe and well-tolerated in adults. However, there are no data in children and adolescents. The aim of the present trial was to assess safety and tolerability of an accelerated dose escalation schedule of an AIT wi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000512561
更新日期:2021-01-27 00:00:00
abstract::Allergic granulomatosis and angiitis was first reported as a disease entity separate from polyarteritis nodosa in 1951 by Churg and Strauss. It is characterized by bronchial asthma, eosinophilia, and vasculitis and is especially rare in women of reproductive age, though, when present, may be associated with fetal mort...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237140
更新日期:1995-10-01 00:00:00
abstract:BACKGROUND:IgE-mediated hypersensitivity to latex has been recognized as an increasing health problem with far-reaching consequences for patients, regarding both their occupational situation and safety in medical care. Therefore, a correct diagnosis of natural rubber latex (NRL) allergy is essential. The purpose of the...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000049518
更新日期:2001-11-01 00:00:00
abstract::The last decade has seen the rapid development of research investigating the mechanisms of apoptosis in a variety of experimental systems. Among the multitude of changes observed in apoptotic cells, chromatin cleavage is considered a biochemical hallmark of apoptosis. Chromatin fragmentation is an enzymatic process wh...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000236778
更新日期:1994-12-01 00:00:00
abstract:BACKGROUND:The population of large parts of Africa, South America and travellers to these areas are at risk of yellow fever (YF) with a 50% mortality risk. Yellow fever vaccine (YFV) propagated in hens' eggs confers protection in 95% of the vaccinated. The rate of anaphylaxis for YFV ranges from 0.42 to 1.8/100,000 dos...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000346350
更新日期:2013-01-01 00:00:00
abstract::Volumetric air sampling was performed at Corpus Christi, Tex., September 1987 to August 1989. Grass aeropollen during all of 1988 accounted for 15.3% of total pollen captured during two major peaks, one in May and another during September and October. The remaining grass pollen, about one-quarter of the total, was fou...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236168
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:Allergic bronchopulmonary aspergillosis (ABPA) develops in the presence of predisposing conditions such as asthma and cystic fibrosis. Even ABPA accompanied by asthma is often complicated by chronic Pseudomonas aeruginosa or nontuberculous mycobacterial infection of the lower respiratory tract, rendering tre...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000507216
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:It is not known why some foods sensitizing via the gastrointestinal tract are prevalent allergenic foods and others are not. Eating habits, processing, and the food matrix have been suggested to influence the allergenicity of a given food. Factors related to protein structure, such as stability to digestion,...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000321200
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:The cytokine transforming growth factor-beta(TGF-beta) has important regulatory roles in the immune system. To investigate the role of intact TGF-beta signaling during the contact hypersensitivity (CHS) response to a respiratory allergen, we exposed Smad3-/- mice to topical trimellitic anhydride (TMA). METH...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236006
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) and food allergy (FA) are common childhood diseases, which may either be interrelated or be the result of skin barrier disruption and gut mucosal dysbiosis. Although some evidence suggests the efficacy of emollients and synbiotics, there is no conclusive evidence on the use of these in...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000501636
更新日期:2019-01-01 00:00:00
abstract::There is increasing evidence that the asthma process is 'driven' and maintained by persistence of a subset of chronically activated T memory cells, sensitized against allergenic, occupational or viral antigens which 'home' to the lung after antigen exposure or viral infection. In general, allergens induce a CD4 T help...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000024049
更新日期:1999-02-01 00:00:00
abstract:BACKGROUND:Intestinal intraepithelial lymphocytes (iIELs) play an important role in intestinal innate immunity and oral immune tolerance. To compare the differences in gene expression between murine iIELs and splenic T lymphocytes, we established the cDNA subtractive library of iIELs and analyzed the iIELs special gene...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000095468
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Shellfish hypersensitivity is amongst the most common food allergies. The major shellfish allergen was identified as tropomyosin. Here, we investigated the immediate hypersensitivity responses, IgE and cell-mediated immune response in mice sensitized with recombinant shrimp tropomyosin. METHODS:Shrimp tropo...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000144038
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Loss-of-function mutations of the filaggrin (FLG) gene cause an impaired skin barrier and increase the risk of atopic dermatitis. Interestingly, FLG mutations have also been found to be associated with a high risk of peanut allergy. OBJECTIVE:We investigated the association of FLG mutations with self-report...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000345949
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The presence of autoantibodies reacting with the high affinity IgE receptor (FcepsilonRIalpha) usually indicates a more severe form of chronic urticaria (CU). Previously, we showed an increased lymphocyte reactivity in CU patients; however, the relation between enhanced cellular immunity and the presence of ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000073719
更新日期:2003-10-01 00:00:00
abstract::Human colostrum and a high molecular weight colostrum fraction (HMWF; > 14,000 D) prevented the adhesion of localized adherent (LA+) O111:H-enteropathogenic Escherichia coli (EPEC) to HeLa cells. This effect was abolished after absorption with an O111:H-LA + EPEC strain, but absorption with a LA- strain of same seroty...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236645
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Activated eosinophils are thought to play an important role in allergic inflammation. Prior reports suggest that eosinophils have receptors recognizing IgA, IgG and IgE; however, little is known regarding the direct effects of antigens and antigen-specific immunoglobulins on the functions of eosinophils. ME...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000320226
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Staphylococcus aureus-producing superantigens (SAgs), such as staphylococcal enterotoxin B (SEB) or toxic shock syndrome toxin-1 (TSST-1), are frequently observed in atopic dermatitis (AD). However, little has been done to establish the association of immune responses to SAgs and the therapeutic response to ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000095298
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Oxidants including reactive oxygen species have been indicated to play an important role in the pathogenesis of asthma. OBJECTIVE:We investigated oxidative status in patients with acute exacerbations of asthma and evaluated the therapeutic response using the D-ROM test which is simple to use and quick. MET...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000126064
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Epinephrine autoinjector (EAI) is prescribed to prevent a fatal outcome in the case of a repeated anaphylactic reactions. We wanted to determine how adult patients who received their first prescription as part of an urgent treatment of an anaphylactic reaction or at their family physician were instructed on ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000513421
更新日期:2021-01-22 00:00:00
abstract::Amino acid sequencing of the major mite allergen Der fII purified from mite body extract revealed that it is a mixture of at least two variants. Substitutions were found only at positions in which amino acid variations were predicted from the nucleotide sequences of three cloned cDNAs. When cDNAs corresponding to the ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236804
更新日期:1994-09-01 00:00:00
abstract::Sensitivity to strong odors has a broad differential diagnosis. A presentation is made of a 60-year-old man with lifelong mild allergic rhinitis and a superimposed 4-year history of sensitivity to smells. He had no response to medical treatments or allergic immunotherapy. His physical examination was unremarkable. Aft...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000316355
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:A new lateral flow test (LFT) for office use provides information about sensitisation to common allergens. Concordance between LFT and skin-prick test (SPT) has not been assessed in epidemiological studies. The aim of this study is to determine LFT-SPT concordance in this field. METHODS:Plasma samples (n = ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000288291
更新日期:2010-01-01 00:00:00
abstract::Studies in migrant populations provide vital opportunities to investigate the role of environmental factors in the pathogenesis of allergic disorders. Differences in allergy prevalence have been observed between migrants and native-born subjects living in the same geographical location. Immigrants who migrate from les...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000494129
更新日期:2019-01-01 00:00:00
abstract::Antibody response after immunization with surface-coupled ovalbumin (OVA) of liposomes was investigated in mice. OVA was coupled to the surface of liposome via amino groups using glutaraldehyde. OVA-liposome conjugate induced a significant anti-OVA IgG antibody production in mice. However, no IgE antibody production s...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237241
更新日期:1996-03-01 00:00:00