Abstract:
BACKGROUND:Transforming growth factor-β1 (TGF-β1)-in-duced proliferation of airway smooth muscle cells plays critical roles in the development of airway remodeling. Six1 (sine oculis homeobox homolog 1) has been demonstrated to be involved in airway inflammation and remodeling in asthmatic mice. OBJECTIVES:The aim of this work was to investigate the potential role of miR-204-5p in the proliferation and extracellular matrix (ECM) production of airway smooth muscle cells in asthma. METHODS:Real-time PCR was used to measure the expression of miR-204-5p in asthmatic airway smooth muscle cells. Cell viability and apoptosis were detected to evaluate the effect of miR-204-5p on airway smooth muscle cells. Dual-luciferase reporter experiments were applied to identify the target genes of miR-204-5p. RESULTS:MiR-204-5p was downregulated notably in asthmatic airway smooth muscle cells as well as cells stimulated with TGF-β1. Overexpression of miR-204-5p markedly suppressed the TGF-β1-induced proliferation of airway smooth muscle cells and the deposition of ECM, whereas the inhibition of miR-204-5p significantly enhanced the proliferation of airway smooth muscle cells and upregulated the level of fibronectin and collagen III. Furthermore, subsequent analyses demonstrated that Six1 was a direct target of miR-204-5p, and Western blot further indicated that miR-204-5p negatively regulated the expression of Six1. Most importantly, the restoration of Six1 expression reversed the inhibitory effect of miR-204-5p on TGF-β1-induced proliferation and ECM production. CONCLUSIONS:MiR-204-5p inhibits TGF-β1-in-duced proliferation and ECM production of airway smooth muscle cells by regulating Six1, identifying a potential therapeutic target for preventing airway remodeling in asthma.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Yang Z,Qu Z,Yi M,Lv Z,Wang Y,Shan Y,Ran N,Liu Xdoi
10.1159/000505064subject
Has Abstractpub_date
2020-01-01 00:00:00pages
239-248issue
4eissn
1018-2438issn
1423-0097pii
000505064journal_volume
181pub_type
杂志文章abstract::T cells are central regulators and mediators of allergic sensitisation and disease. The house dust mite allergens are a biochemically diverse group of proteins which are present in extracts and the environment in a wide range of concentrations. Here the importance of ascertaining the contribution of the different alle...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000023824
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:Cry j 1 and Cry j 2 are thought to be the major allergens of Japanese cedar pollen. HLA class II types capable of presenting T-cell epitopes in both allergens and their role in induction of T-cell subsets are not well known. METHODS:CD4+ T (Th)-cell clones (TCCs) specific to either Cry j 1 or Cry j 2 were g...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024194
更新日期:1999-07-01 00:00:00
abstract:BACKGROUND:The importance of fungal allergens in the development of allergic diseases in a desert environment is uncertain. This study evaluated the prevalence of IgE sensitization to moulds among patients with allergic respiratory diseases in Kuwait - a desert country. METHODS:A total of 810 patients (male:female rat...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024343
更新日期:2000-04-01 00:00:00
abstract:BACKGROUND:We reported on the constitutive interferon-gamma receptor (IFN-gammaR) expression on eosinophils. But signal transduction through IFN-gammaR on eosinophils remains to be elucidated. In this study, we examined the involvement of the Jak/Stat pathway in the signaling of eosinophils after IFN-gammaR conjugation...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024159
更新日期:1999-02-01 00:00:00
abstract:BACKGROUND:Previous studies have shown a negative association between ACP1 *B/*C genotype and total IgE level. ACP1 (acid phosphatase locus 1) is a polymorphic phosphotyrosine phosphatase that interacts with IL4-RA and is involved in T cell receptor signaling. METHODS:In the present paper, we have studied the relation...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000107466
更新日期:2008-01-01 00:00:00
abstract::Nitric oxide (NO) is a potent biological mediator, and has a regulatory role in a wide variety of cellular and tissue functions. In the upper and lower airways, NO has been suggested to be involved in different functions with regulatory, protective, defensive or damaging effects. It is obvious that NO plays an importa...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000324678
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:The role of food allergens in the induction of allergic reactions in the airways is not completely understood. The aim of the present study was to evaluate fluorocytometric assays of the peripheral blood during food challenge tests in children with asthma and food allergy. PATIENTS AND METHODS:22 children w...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000226239
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Accumulating evidence indicates that eotaxin is the primary mediator of tissue eosinophilia. In the present study, we analyzed the mechanisms of eotaxin generation by Th1-/Th2-derived cytokines in vitro. METHODS:Human dermal fibroblasts, human umbilical vein endothelial cells and A549 human bronchial epithe...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053634
更新日期:2000-05-01 00:00:00
abstract::The prevalence of IgE myeloma is 0.01% in all plasmocytomas. Although signs and symptoms are similar, it is generally accepted that IgE myeloma has a more malignant clinical course than other forms of plasmocytoma with monoclonal gammopathy. The disease has widely variable presentation. Most of the reported cases were...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000237490
更新日期:1997-04-01 00:00:00
abstract::We aimed to evaluate the therapeutic effect of nebulized beclomethasone dipropionate (nBDP) on both allergic asthma and rhinitis. In a randomized, double-blind, placebo-controlled study, 40 children (mean age 10.7 ± 2.1 years) with allergic asthma and rhinitis received either nBDP (daily dose of 800 µg, administered t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000343137
更新日期:2013-01-01 00:00:00
abstract::Because of a unique combination of challenges facing human immunodeficiency virus vaccine developers, a number of traditional and novel vaccine designs are being evaluated essentially in parallel. Monomeric proteins, poxvirus vectors, peptides, and particle-based candidate vaccines have entered or will soon enter huma...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000237173
更新日期:1995-12-01 00:00:00
abstract:BACKGROUND:Mast cell tryptase has recently been reported to be involved in atherosclerotic plaque destabilization. However, the results of these reports are conflicting. METHODS:The aim of this study was to characterize the role of tryptase as a prognostic marker of patient cardiovascular complexity in acute coronary ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000360164
更新日期:2014-01-01 00:00:00
abstract::Elevated levels of ovine reaginic antibody were induced by immunization with Ascaris suum antigens. The PCA activity of this antibody persisted in the skin of sheep for 20 days and was abolished by heating to 56 degrees C, suggesting that it was immunoglobulin E. Attempts to isolate IgE from this hyperimmune serum by ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236479
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Reports on oral immunotherapy (OIT) for anaphylactic food allergy are lacking. We investigated the efficacy and safety of peanut OIT for anaphylactic patients. METHODS:We enrolled 22 peanut anaphylactic patients who underwent OIT between 2011 and 2013, all of whom demonstrated anaphylaxis during a baseline ...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000486310
更新日期:2018-01-01 00:00:00
abstract::The aim of this study was to investigate whether repeated exposure to subclinical doses of cat allergens, not inducing asthma symptoms, could affect eosinophil cationic protein (ECP) levels in bronchoalveolar lavage (BAL) or in peripheral blood, without the appearance of clinical symptoms. Twelve patients with mild as...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024234
更新日期:1999-10-01 00:00:00
abstract:BACKGROUND:The allergenic potential of chicken egg white ovomucoid (OVM) is thought to depend on its stability to heat treatment and digestion. Pepsin-digested fragments have been speculated to continue to exert an allergenic potential. OVM was digested in simulated gastric fluid (SGF) to examine the reactivity of the ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000082581
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:To understand how the high-affinity IgE receptor (FcepsilonRI) communicates with downstream effectors, we focused on exploring the functional importance of the FcepsilonRI-mediated formation and localization of a signaling complex that contains the hematopoietic cell-specific scaffolding protein linker for a...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000053692
更新日期:2001-01-01 00:00:00
abstract::Accumulation and activation of eosinophils in tissue are critical events in the allergic inflammatory response and adhesion molecules play important roles in this process. We previously demonstrated that human eosinophils expressed a nuclear receptor, peroxisome proliferator-activated receptor gamma (PPARgamma), and t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000126054
更新日期:2008-01-01 00:00:00
abstract:INTRODUCTION:Pathologic accumulation and activation of mast cells and eosinophils are implicated in allergic and inflammatory diseases. Sialic acid-binding immunoglobulin-like lectin (Siglec)-8 is an inhibitory receptor selectively expressed on mast cells, eosinophils and, at a lower extent, basophils. When engaged wit...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000501637
更新日期:2019-01-01 00:00:00
abstract::We examined the capacity of interleukin-4 (IL) production from lymphocytes and basophils, isolated from the peripheral blood of allergic patients sensitive to house dust mite, after stimulation with mite extract. IL-4 production was measured by a sensitive bioassay based on coculture with CT.h4S (a human IL-4-responsi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237375
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND:Farming has been widely reported to be associated with decreased risk of developing atopic disorders, but underlying immunomodulatory mechanisms are still not fully defined. We delineated T-cell functions after induction of mucosal tolerance in the context of intranasally delivered organic dust compounds, li...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000128583
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:In some subjects, specific foods trigger anaphylaxis when exercise follows ingestion (specific food-dependent exercise-induced anaphylaxis, FDEIAn). Skin test and/or RAST positivity to foods suggest an IgE-mediated pathogenic mechanism. Others suffer from anaphylaxis after all meals followed by exercise, reg...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053825
更新日期:2001-07-01 00:00:00
abstract::A 20mer peptide representing the N-terminus of a major allergen, Alt a I, of Alternaria alternata was synthesized and examined for its antibody binding and antibody induction activities. Alt a I peptide-BSA conjugate reacted with both human IgE and rabbit anti-Alt a I IgG in ELISA, albeit the binding of the peptide to...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237161
更新日期:1995-11-01 00:00:00
abstract::Mast cells are recognized not only as the major effector cells of type I hypersensitivity reactions but also as an important player of innate immune response against bacterial infection. Type I IFNs are also involved in the response against bacterial infection. However, the role of type I IFNs and their associated Jan...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000077789
更新日期:2004-06-01 00:00:00
abstract:BACKGROUND:Recombinant allergens are preferred over natural allergen extracts in measuring antibodies. We tested the use of recombinant variants of the major mouse allergen Mus m 1 in detection of mouse-specific antibodies in sera of laboratory animal workers and children. METHODS:Six recombinant major urinary protein...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000106318
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Mast cells (MCs) are multifunctional effector cells of the immune system. These cells originate from pluripotent hemopoietic progenitors. In contrast to basophils and other leukocytes, MCs exhibit a remarkably long life span (years) in vivo. Although a role for stem cell factor (SCF) and SCF receptor (KIT) i...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000066773
更新日期:2002-11-01 00:00:00
abstract:BACKGROUND:Diethylhexyl phthalate (DEHP) and diisononyl phthalate (DINP), two commonly used plasticizers in flexible polyvinylchloride formulations, have potentially adverse effects on human health. However, the influence of these diphthalates on allergic responses remains unclear. In this study we examined the effects...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000078768
更新日期:2004-07-01 00:00:00
abstract::A high-dose, accelerated escalation schedule during subcutaneous allergen-specific immunotherapy (AIT) is safe and well-tolerated in adults. However, there are no data in children and adolescents. The aim of the present trial was to assess safety and tolerability of an accelerated dose escalation schedule of an AIT wi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000512561
更新日期:2021-01-27 00:00:00
abstract:BACKGROUND:Depression and smoking contribute to the prognosis of autoimmune rheumatoid arthritis (RA). Advanced glycation end products (AGEs) are also detected in RA patients. This study correlates RA in patients with various levels of depression and a history of smoking through the detection of antibodies against AGEs...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000489896
更新日期:2018-01-01 00:00:00
abstract::Human colostrum and a high molecular weight colostrum fraction (HMWF; > 14,000 D) prevented the adhesion of localized adherent (LA+) O111:H-enteropathogenic Escherichia coli (EPEC) to HeLa cells. This effect was abolished after absorption with an O111:H-LA + EPEC strain, but absorption with a LA- strain of same seroty...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236645
更新日期:1994-01-01 00:00:00