Abstract:
BACKGROUND:To understand how the high-affinity IgE receptor (FcepsilonRI) communicates with downstream effectors, we focused on exploring the functional importance of the FcepsilonRI-mediated formation and localization of a signaling complex that contains the hematopoietic cell-specific scaffolding protein linker for activation of T cells (LAT) and the guanine nucleotide exchange factor Vav1. METHODS:Using the mast cell line RBL-2H3, we explored the localization of these proteins by confocal microscopy and cell fractionation. Additionally, the mechanism of function and the importance of LAT and Vav1 to mast cells was studied in genetically disrupted mice and in mast cells derived from their bone marrow. RESULTS:We found that LAT, Vav1 and the adapter molecule SLP-76 associated in detergent-resistant microdomains (lipid rafts) found in the plasma membrane upon FcepsilonRI stimulation. In the absence of LAT, mast cells showed a remarkable loss of the secretory response and reduced cytokine responses. Vav1 deficiency also affected secretion, although not to the extent of LAT deficiency, and inhibited IL-2 and IFN-gamma production. LAT- and Vav1-deficient mice showed reduced blood histamine levels after a systemic anaphylaxis challenge as compared to their normal counterparts. CONCLUSIONS:The results demonstrate that LAT is a central mediator in IgE receptor signaling by regulating multiple signaling pathways that affect mast cell degranulation and cytokine production. Vav1, a component of this LAT-containing signaling complex, regulates a specific subset of these responses.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Rivera J,Arudchandran R,Gonzalez-Espinosa C,Manetz TS,Xirasagar Sdoi
10.1159/000053692keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
137-41issue
1-3eissn
1018-2438issn
1423-0097pii
53692journal_volume
124pub_type
杂志文章,评审abstract:BACKGROUND:Antiphospholipid antibodies (aPL) show great heterogeneity. Different phospholipids, with or without protein cofactor(s), and phospholipid binding proteins alone have been proposed as the target molecules for aPL. In order to determine the influence of phospholipid degradation products on the binding of aPL,...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237426
更新日期:1997-01-01 00:00:00
abstract::Ever since the realization that T lymphocytes are key players in the defense against pathogens and tumors, a major aim of immunologists has been to understand the relationship between the functional and migratory properties of antigen-specific T cells. The current paradigm proposes that T cells follow organ-specific t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000074896
更新日期:2003-12-01 00:00:00
abstract::Monocytes and lymphocytes from human blood contain 0.043 +/- 0.007 and 0.053 +/- 0.014 pg histamine/cell, respectively, which can be released by a number of stimulants (A 23187, C5a, substance P, specific allergen). The release process takes 60-120 min to reach its end point, in contrast to tissue mast cells which com...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236974
更新日期:1995-05-01 00:00:00
abstract::Thymulin is a nonapeptide hormone isolated from the thymus gland. It has immunomodulatory effects which have not yet been well defined. Its major actions have been shown to be on T-cells and their immature precursors. In this study, thymulin was tested in vitro for its effect on the release of IL-1 alpha, IL-2, IL-6 a...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236509
更新日期:1993-01-01 00:00:00
abstract::The prevalence of IgE myeloma is 0.01% in all plasmocytomas. Although signs and symptoms are similar, it is generally accepted that IgE myeloma has a more malignant clinical course than other forms of plasmocytoma with monoclonal gammopathy. The disease has widely variable presentation. Most of the reported cases were...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000237490
更新日期:1997-04-01 00:00:00
abstract::A case of anaphylaxis following a bite from a Siberian hamster (SH; Phodopus sungorus) is described. Skin prick tests with hair, urine and salivary gland extracts from SH were positive, while the tests were negative for hair extracts from other rodents. IgE immunoblotting with the patient serum revealed 3 IgE-binding ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000324956
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:The clinical significance and efficacy of treating patients who have immunoglobulin (Ig) G subclass deficiency and/or antibody deficiency with Ig-replacement therapy has been debated. There are no clear guidelines to recommend intravenous gammaglobulin (IgIV) in these patients as there are few published stud...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000199723
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Volatile monoisocyanates are formed through thermal degradation when products containing polyurethane are heated. Repeated exposure to diisocyanates, such as toluene diisocyanate (TDI) are a well-known cause of occupational asthma. However, although monoisocyanates are abundant in occupational settings, ther...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000222675
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Ultra-rush regimens for administering sublingual immunotherapy to patients with allergies are becoming more widespread. We aimed to assess treatment safety for patients with allergic rhinitis with or without asthma caused by Dermatophagoides house dust mites. METHODS:This observational study at 5 Spanish ce...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1159/000319211
更新日期:2011-01-01 00:00:00
abstract::The morbidity and mortality of elderly onset asthma have recently been on the increase. To evaluate the allergic status of the elderly onset asthmatics, we performed skin tests for allergens and estimated serum total IgE levels of the patients. The proportion of the skin-test-positive patients among the elderly onset ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053600
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) is increasing among the elderly. Staphylococcus aureus colonization is one of the most important aggravating factors in AD. OBJECTIVE:This study analyses the clinical features of AD in elderly patients and determines the pathogenic roles of staphylococcal superantigens in AD with low ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000329150
更新日期:2012-01-01 00:00:00
abstract::Drug hypersensitivity reactions (DHR) are clinically and functionally heterogeneous. Different subclassifications based on timing of symptom appearance or type of immune mechanism have been proposed. Here, we show that the mode of action of drugs leading to immune/inflammatory cell stimulation is a further decisive fa...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000453265
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Aspirin-intolerant asthma (AIA) is a clinical syndrome characterized by acute bronchoconstriction following the ingestion of aspirin. Solute carrier family 22, member 2 (SLC22A2), also known as organic cation transporter 2 (OCT2), is predominantly expressed in the luminal membrane of airway epithelial cells ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000321267
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Animal models are used to mimic human asthma, however, not all models replicate the major characteristics of the human disease. Spontaneous development of asthma with hallmark features similar to humans has been documented to occur with relative frequency in only one animal species, the cat. We hypothesized ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000080654
更新日期:2004-10-01 00:00:00
abstract:BACKGROUND:The widespread house ant, Monomorium pharaonis (pharaoh ant, PA), was recently identified as a potential cause of respiratory allergies. However, there are no reports of the distribution of PA allergens in various environments. We developed specific ELISA inhibition assays and measured the distribution and a...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000103999
更新日期:2007-01-01 00:00:00
abstract::Ovalbumin (OVA)-pullulan conjugates were made using four different conjugation methods and eight different molecular weights of pullulan ranging from 4,200 to 600,000. Pretreatment of mice by the administration of conjugates made by using cyanulic chloride or cyanogen bromide and pullulan of molecular weight 40,000 or...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236536
更新日期:1993-01-01 00:00:00
abstract::The 3-dimensional structure of an allergen defines the accessible parts on the surface of the molecule or epitopes that interact with antibodies. Mapping the antigenic determinants for IgE antibody binding has been pursued through strategies based on the use of overlapping synthetic peptides, recombinant allergenic fr...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000260078
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:To elucidate the usefulness of antigen-specific immunotherapy based on oral vaccination with an edible part of the plant, we examined the effect of transgenic (Tg) rice seeds expressing an immunodominant fragment of the group 1 antigen of Dermatophagoides pteronyssinus (Der p 1) on a murine model of asthma. ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000210649
更新日期:2009-01-01 00:00:00
abstract::To determine the variation of antigenic water soluble proteins in white birch (Betula pendula) pollen, extracts of pollen from different sides of individual trees were analyzed by isoelectric focusing (IEF), crossed immunoelectrophoresis, and crossed radioimmunoelectrophoresis. IgE-antibody-binding patterns were also ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237184
更新日期:1995-12-01 00:00:00
abstract:BACKGROUND:Grape allergy is considered rare; grape lipid transfer protein (LTP; Vit v 1), an endochitinase and a thaumatin-like protein (TLP) have been reported as grape allergens. A considerable number of patients have referred to our department for severe reactions to grapes, and several IgE binding proteins were det...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000098657
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Exposure to the house dust mite Blomia tropicalis is increasingly being implicated as a major risk factor for asthma exacerbations in sensitized individuals. The objective of this study is to clone and characterize B. Tropicalis allergens in order to better define their role in allergic asthma. METHODS:A la...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000068375
更新日期:2003-01-01 00:00:00
abstract::Adhesion molecules of microvascular endothelial cells play a key role in the inflammatory processes involved in nonallergic sinusitis. We investigated the cytokine-regulated expression of intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1), and the effect of dexamethas...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000023992
更新日期:1998-09-01 00:00:00
abstract::Recently, the induction of T cell anergy has been considered as a sophisticated form of immunotherapy. However, the multiplicity of T cell allergen epitopes discourages the practical use of this method. Since orally administered antigen proteins are digested to peptides and absorbed into the circulation, oral administ...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000237002
更新日期:1995-05-01 00:00:00
abstract:BACKGROUND:One hundred years ago, Noon [Lancet 1911;1:1572-1573], using conjunctival provocation testing (CPT), was the first to demonstrate the effectiveness of subcutaneous immunotherapy (SCIT) in grass-allergic subjects with hay fever. In this centenary year, we present data that, by use of CPT and allergen-specific...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000330649
更新日期:2012-01-01 00:00:00
abstract::The low responsiveness of contact sensitivity (CS) in sensitized and challenged mice was induced by using hapten-amino acid derivatives. Subcutaneous injection of trinitrophenyl (TNP)-lysine or oxazolone-glycine inhibited ear swelling responses in mice that received rechallenge. The inhibitory effect was long-lasting ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236556
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Numerous strategies have been proposed for the treatment of peanut allergies, but despite the steady advancement in our understanding of atopic immune responses and the increasing number of deaths each year from peanut anaphylaxis, there is still no safe, effective, specific therapy for the peanut-sensitive ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053672
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Th17 is a subset of T-helper lymphocytes that produce proinflammatory cytokines, mainly IL-17. Serum IL-17 is increased in allergic patients and relates to clinical severity. Recently, it has been reported that CD161 is a highly upregulated gene in Th17 clones and all IL-17-producing cells are contained in C...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000330903
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Immunoglobulin G4-related disease (IgG4-RD) is a systemic immunological disorder characterized by fibro-inflammatory conditions; however, the pathobiology of IgG4-RD has not been fully identified. OBJECTIVE:This study aimed to analyze systemic differences of innate and adaptive immune cells from healthy con...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000508699
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:A growing body of evidence suggests that proinflammatory cytokines play a role in allergic inflammation by attracting and activating inflammatory cells. In this study, we have investigated the relationship between interleukin-8 (IL-8) in nasal lavage fluid and the local activation of eosinophils and neutroph...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000023925
更新日期:1998-05-01 00:00:00
abstract:BACKGROUND:Sweating can worsen atopic dermatitis (AD). The purpose of this work was to study the associations between reactivity to autologous sweat and the clinical severity of AD as well as investigate the possible wheal-inducing factors of sweat. METHODS:Intracutaneous skin tests with autologous sweat were performe...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000447638
更新日期:2016-01-01 00:00:00