Abstract:
:Elevated levels of ovine reaginic antibody were induced by immunization with Ascaris suum antigens. The PCA activity of this antibody persisted in the skin of sheep for 20 days and was abolished by heating to 56 degrees C, suggesting that it was immunoglobulin E. Attempts to isolate IgE from this hyperimmune serum by gel filtration, ion exchange and affinity chromatography resulted in the preparation of a PCA-positive fraction containing proteins with molecular weights of 70, 56 and 22 kD on SDS-PAGE. This preparation was used to raise an antiserum in a rabbit to IgE which was rendered specific by absorption. An antiserum to the heavy chain of ovine IgE was also raised in a rabbit by immunization with a fraction prepared from the 68- to 80-kD region of SDS-PAGE by excision and electroelution. Both antisera were positive in reverse cutaneous anaphylaxis tests and recognized a single protein with a molecular weight of 70-72 kD on SDS-PAGE immunoblotting. The presence of this protein in reaginic sheep serum, the molecular weight of its heavy chain, its heat lability and long-term skin-sensitizing ability are characteristic of IgE.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Yilmaz H,Roe JM,Morgan KLdoi
10.1159/000236479subject
Has Abstractpub_date
1993-01-01 00:00:00pages
369-75issue
4eissn
1018-2438issn
1423-0097journal_volume
101pub_type
杂志文章abstract::Two cDNA clones named P9* and P1* of 794 and 631 bp, respectively, were isolated from a lambda ZAP cDNA expression library using Parietaria judaica (Pj) pollen-specific IgE antibodies from a pool of sera (n = 23) of patients allergic to Pj. Sequence analysis showed open reading frames of 176 and 138 amino acids. Both ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237479
更新日期:1997-04-01 00:00:00
abstract:BACKGROUND:The cytokine transforming growth factor-beta(TGF-beta) has important regulatory roles in the immune system. To investigate the role of intact TGF-beta signaling during the contact hypersensitivity (CHS) response to a respiratory allergen, we exposed Smad3-/- mice to topical trimellitic anhydride (TMA). METH...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236006
更新日期:2010-01-01 00:00:00
abstract::There is increasing evidence that the asthma process is 'driven' and maintained by persistence of a subset of chronically activated T memory cells, sensitized against allergenic, occupational or viral antigens which 'home' to the lung after antigen exposure or viral infection. In general, allergens induce a CD4 T help...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000024049
更新日期:1999-02-01 00:00:00
abstract:BACKGROUND:Exposure to Aspergillus fumigatus allergens results in the sensitization and the development of allergic bronchopulmonary aspergillosis in susceptible individuals. Aspergillus antigen consists of a number of chemically diverse components and their cumulative or synergistic effect may result in disease. When ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000065882
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:There seems to be an association between paracetamol consumption during late pregnancy and the prevalence of wheezing in infancy and childhood. The aim of the present study is to determine whether the aforementioned association is modified by the presence of asthma in the mother. METHODS:A total of 1,741 ch...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000176304
更新日期:2009-01-01 00:00:00
abstract::Psoriasis (Ps) is a chronic, immune-mediated, skin inflammatory disease affecting up to 3% of the population worldwide. Different environmental triggers initiate this complex multifactorial syndrome. Many individuals affected by Ps (6-26%) develop inflammatory disease in other organs, often in the joints as in psorias...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000375401
更新日期:2015-01-01 00:00:00
abstract::Phosphoinositide 3-kinases (PI3Ks) are known to be involved in a variety of cellular responses such as cell survival, proliferation, differentiation and cell migration. Recently, PI3Ks have been associated with the pathogenesis of asthma because various immune cells regulate allergic responses. Among the three classes...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000312132
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:It has been shown that antigen presentation by resting B cells can induce tolerance to intravenously administered protein antigens, but the role of resting B cells in the induction of oral tolerance is unclear. METHODS:Mice continuously treated since birth with rabbit anti-mouse IgM serum for 5 weeks were d...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024394
更新日期:2000-07-01 00:00:00
abstract::Red soft coral (RSC; Dendronephthya nipponica, a marine coelenterate) causes spiny lobster fishermen living along the Pacific coast of Miyazaki Prefecture in Japan to develop occupational allergies, such as conjunctivitis, rhinitis, dermatitis and bronchial asthma. The aim of this study was to purify and to characteri...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053807
更新日期:2001-06-01 00:00:00
abstract:BACKGROUND:Grape allergy is considered rare; grape lipid transfer protein (LTP; Vit v 1), an endochitinase and a thaumatin-like protein (TLP) have been reported as grape allergens. A considerable number of patients have referred to our department for severe reactions to grapes, and several IgE binding proteins were det...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000098657
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Nuclear factor (NF)-kappaB is a transcription factor known to regulate allergy-associated cytokine and chemokine production related to the induction of inflammation. I kappaB kinase beta (IKK beta), which is responsible for activation of the NF-kappaB pathway, may be an ideal molecular target to inhibit this...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000161579
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:L-selectin (CD62L) and Mac-1 (CD11b/CD18) play a crucial role in the infiltration of eosinophils. However, changes in CD62L and CD11b expression on eosinophils after stimulation with cytokines were little studied. METHODS:Eosinophils in peripheral blood of healthy volunteers were purified and cultured with ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053590
更新日期:1999-01-01 00:00:00
abstract::A case of anaphylaxis following a bite from a Siberian hamster (SH; Phodopus sungorus) is described. Skin prick tests with hair, urine and salivary gland extracts from SH were positive, while the tests were negative for hair extracts from other rodents. IgE immunoblotting with the patient serum revealed 3 IgE-binding ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000324956
更新日期:2012-01-01 00:00:00
abstract::The role of CD8+ T cells in the regulation of IgE was investigated by in vivo CD8+ T-cell depletion. Following intraperitoneal immunization with ovalbumin (OVA), OVA-specific T cell responses were first detected in the draining lymph nodes (LN) and subsequently in the spleen. In vitro depletion of LN CD8+ cells reduce...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237534
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND:Ultra-rush regimens for administering sublingual immunotherapy to patients with allergies are becoming more widespread. We aimed to assess treatment safety for patients with allergic rhinitis with or without asthma caused by Dermatophagoides house dust mites. METHODS:This observational study at 5 Spanish ce...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1159/000319211
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Sensitivity to the mite Blomia tropicalis is related to asthma in tropical climates, but correlates of sensitivity to B. tropicalis and its relationship to Dermatophagoides pteronyssinus sensitivity have not been widely examined in families with asthma. The main objective of this study was to determine preva...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000070927
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:We analyzed the T cell activation potency and the IgE-binding properties (allergenicity) of nine isoforms of Bet v 1, the major allergen of birch pollen. METHODS:The capacity of recombinant Bet v 1 isoforms to bind serum IgE from allergic patients was evaluated by immunoblot experiments and skin prick tests...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237524
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND:Subcutaneous immunotherapy for respiratory allergy has shown a long lasting efficacy after its discontinuation, whereas evidence in the case of sublingual immunotherapy (SLIT) is weak. This retrospective study evaluates whether SLIT exerts a long-lasting effect and whether it relates to its duration. METHOD...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000096001
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:The fractional concentration of exhaled nitric oxide (FeNO) is a surrogate biomarker for Th2-dependent bronchial inflammation. The present study investigated whether FeNO may characterize allergic rhinitis (AR) patients. METHODS:A total of 553 AR patients (497 males, mean age 28.8 years) were evaluated. Tho...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000456548
更新日期:2017-01-01 00:00:00
abstract::Profilins are ubiquitous proteins, present in all eukaryotic cells and identified as allergens in pollen, latex and plant foods. The highly conserved structure justifies the cross-reactive nature of IgE antibodies against plant profilins and their designation as pan-allergens. Primary sensitization to profilin seems t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000321178
更新日期:2011-01-01 00:00:00
abstract::Recent data suggest that stem cell factor (SCF or c-kit ligand, KL) is a major regulator of human mast cells (MCs). In the present study, MCs derived from the lung (n = 8), uterus (n = 14) and heart (n = 4) were analyzed for expression of c-kit receptor and for responses to recombinant SCF. MCs of all organs tested we...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236568
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Little information is available on eosinophil activation and the cytokine profile in virus-induced acute exacerbation of bronchial asthma; therefore, we examined the effects of treatments that included systemic corticosteroids on serum eosinophil cationic protein (ECP) and 17 cytokines/chemokines in rhinovir...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000327434
更新日期:2011-01-01 00:00:00
abstract::Ever since the realization that T lymphocytes are key players in the defense against pathogens and tumors, a major aim of immunologists has been to understand the relationship between the functional and migratory properties of antigen-specific T cells. The current paradigm proposes that T cells follow organ-specific t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000074896
更新日期:2003-12-01 00:00:00
abstract:BACKGROUND:Nitrosative and acid stress play an important role in the pathogenesis of asthma. The aim of this study was to evaluate whether, in asthmatics, a link exists between the concentrations of nitrite/nitrate, ammonia and pH values in exhaled breath condensate (EBC) and asthma severity, lung function, exhaled nit...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000335674
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Recently, the relationship between antigen contact via skin (skin sensitization) and the development of food allergies has gained increasing attention. However, few studies have examined the effects of skin sensitization on healthy skin. OBJECTIVE:To examine the effect of sensitization in healthy skin on Ig...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000511239
更新日期:2020-11-11 00:00:00
abstract::There is a great need to develop a method for making an accurate and reliable in vitro diagnosis of adverse hypersensitivity reactions to drugs. We measured the amount of sulfidoleukotriene (sLT) released from the peripheral blood leukocytes obtained from 25 patients who developed hypersensitivity reactions following ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237695
更新日期:1997-12-01 00:00:00
abstract:BACKGROUND:Transforming growth factor-β1 (TGF-β1)-in-duced proliferation of airway smooth muscle cells plays critical roles in the development of airway remodeling. Six1 (sine oculis homeobox homolog 1) has been demonstrated to be involved in airway inflammation and remodeling in asthmatic mice. OBJECTIVES:The aim of ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000505064
更新日期:2020-01-01 00:00:00
abstract::Bed bugs may cause mild to severe cutaneous reactions. We studied the ability of bed bug salivary extract (SGE) to induce inflammatory reactions responsible for cutaneous manifestations and found that SGE stimulated the production of several potent chemokines and cytokines from macrophages. Chemokines induced by SGE i...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000345134
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:ADAM (a disintegrin and metalloprotease) family members, characterized by a metalloprotease and a disintegrin domain, are membrane-anchored glycoproteins involved in proteolysis and cell adhesion. ADAM8 might have an important role in allergic inflammation. It can cleave a variety of substrates and is a shed...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000097359
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Aspirin-intolerant asthma (AIA) is a clinical syndrome characterized by acute bronchoconstriction following the ingestion of aspirin. Solute carrier family 22, member 2 (SLC22A2), also known as organic cation transporter 2 (OCT2), is predominantly expressed in the luminal membrane of airway epithelial cells ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000321267
更新日期:2011-01-01 00:00:00