Abstract:
:Recent data suggest that stem cell factor (SCF or c-kit ligand, KL) is a major regulator of human mast cells (MCs). In the present study, MCs derived from the lung (n = 8), uterus (n = 14) and heart (n = 4) were analyzed for expression of c-kit receptor and for responses to recombinant SCF. MCs of all organs tested were recognized by mAbs to c-kit (YB5.B8, SR-1) as assessed by combined toluidine blue/immunofluorescence staining. Activation by rhSCF (10 ng/ml, 60 min) resulted in histamine release from lung MCs (SCF 12.8 +/- 2.7% histamine release; control 2.8 +/- 0.8%, p < 0.01), uterus MCs (SCF 16.8 +/- 5.8%; control 5.2 +/- 2.5%, p < 0.01) and heart MCs (SCF 18.4 +/- 2.6%; control 1.7 +/- 0.23%, p < 0.01). Short-term pre-incubation with rhSCF (15 min) did not result in histamine secretion (p > 0.05), but in an increase (lung 2.4 +/- 1.0 fold; uterus 2.1 +/- 1.1 fold, and heart 2.0 +/- 0.4 fold) of alpha IgE-induced mediator release (p < 0.05). The effects of SCF were dose-dependent (maximum responses at 10-100 ng/ml) and dependent on extracellular calcium. A monoclonal antibody to SCF was found to inhibit the effects of SCF on MCs. Furthermore, MCs could be desensitized specifically by pre-incubation of MCs with rhSCF in Ca-free medium. Together, these data suggest that SCF triggers mediator secretion from MCs in various organs via binding to the c-kit receptor.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Sperr WR,Czerwenka K,Mundigler G,Müller MR,Semper H,Klappacher G,Glogar HD,Lechner K,Valent Pdoi
10.1159/000236568subject
Has Abstractpub_date
1993-01-01 00:00:00pages
170-5issue
2eissn
1018-2438issn
1423-0097journal_volume
102pub_type
杂志文章abstract:BACKGROUND:High osmolal ionic radiocontrast media (RCM) cause vascular release of endothelin-1 (ET-1) and activate mast cells. Iomeprol, a nonionic RCM, has recently been reported not to activate cardiac mast cells. This coronary angiography study was performed to extend those findings using another nonionic RCM, iopro...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000070435
更新日期:2003-05-01 00:00:00
abstract:BACKGROUND:15-Deoxy-Delta(12,14)-prostaglandin J2 (15d-PGJ2), a major prostanoid metabolized from prostaglandin D2 (PGD2), plays an important role in various biological processes including inflammatory responses. 15d-PGJ2 exerts its effects through two major receptors, chemoattractant receptor- homologous molecule expr...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000211377
更新日期:2009-01-01 00:00:00
abstract::The downstream signaling components of high-affinity IgE receptor (FcepsilonRI) were studied using yeast two-hybrid screening of the cDNA library constructed from RBL-2H3 cells. The cytoplasmic part of the gamma-chain but not that of the beta-chain was found to interact with pyruvate kinase in the yeast. The in-vitro-...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024183
更新日期:1999-06-01 00:00:00
abstract:BACKGROUND:Cross-sectional epidemiological studies have demonstrated that farm milk from traditional farm settings possesses allergoprotective properties. Up to now, it has not been clarified which milk ingredient is responsible for protection against allergic diseases. As farm milk is rich in conjugated linoleic acids...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000358523
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:We reported on the constitutive interferon-gamma receptor (IFN-gammaR) expression on eosinophils. But signal transduction through IFN-gammaR on eosinophils remains to be elucidated. In this study, we examined the involvement of the Jak/Stat pathway in the signaling of eosinophils after IFN-gammaR conjugation...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024159
更新日期:1999-02-01 00:00:00
abstract::T cells are central regulators and mediators of allergic sensitisation and disease. The house dust mite allergens are a biochemically diverse group of proteins which are present in extracts and the environment in a wide range of concentrations. Here the importance of ascertaining the contribution of the different alle...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000023824
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:Numerous strategies have been proposed for the treatment of peanut allergies, but despite the steady advancement in our understanding of atopic immune responses and the increasing number of deaths each year from peanut anaphylaxis, there is still no safe, effective, specific therapy for the peanut-sensitive ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053672
更新日期:2001-01-01 00:00:00
abstract::A case of anaphylaxis following a bite from a Siberian hamster (SH; Phodopus sungorus) is described. Skin prick tests with hair, urine and salivary gland extracts from SH were positive, while the tests were negative for hair extracts from other rodents. IgE immunoblotting with the patient serum revealed 3 IgE-binding ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000324956
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:There seems to be an association between paracetamol consumption during late pregnancy and the prevalence of wheezing in infancy and childhood. The aim of the present study is to determine whether the aforementioned association is modified by the presence of asthma in the mother. METHODS:A total of 1,741 ch...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000176304
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Serum-derived hyaluronan (HA)-associated proteins (SHAPs), the heavy chains of inter-α-trypsin inhibitor, covalently bind to HA to form the SHAP-HA complex. The SHAP-HA complex is involved in the pathophysiology of inflammatory diseases, including rheumatoid arthritis. We investigated whether this complex is...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000314362
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND AND AIM:Although still controversial, drug provocation tests (DPTs) are considered by many as the gold standard for diagnosis of drug hypersensitivity. No studies have addressed the opinion of patients submitted to DPT. We aimed to determine patient satisfaction with diagnostic DPT and their perception of it...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,多中心研究
doi:10.1159/000323898
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Reports on oral immunotherapy (OIT) for anaphylactic food allergy are lacking. We investigated the efficacy and safety of peanut OIT for anaphylactic patients. METHODS:We enrolled 22 peanut anaphylactic patients who underwent OIT between 2011 and 2013, all of whom demonstrated anaphylaxis during a baseline ...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000486310
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:T(H)2 inflammation and bronchial smooth muscle cell (BSMC) hyperplasia are characteristic features of asthma, but whether these phenomena are linked remains unknown. This study aims to define the effect of the T(H)2 cytokines IL-4 and IL-13 on human BSMC proliferation when administered alone or in combinatio...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000113517
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Studies comparing skin prick test (SPT) devices have revealed varying results in performance and there is little known about their use on children. METHODS:We performed 2 complementary studies to test the sensitivity, reproducibility and acceptability of commercially available SPT devices (Stallerpoint, Ant...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000350788
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Epidemiological data on food allergy are scarce in African countries. We studied the prevalence of food sensitization in Ghanaian schoolchildren. METHODS:Children (5-16 years; n = 1,714) from 9 Ghanaian schools were given parental consent to participate in the study. Adverse reactions and food consumption w...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000318704
更新日期:2011-01-01 00:00:00
abstract::In this study, we postulated that repeated cycles of IgE passive sensitisation and antigen challenge may play a role in up-regulating eosinophil response in allergic conditions. Antigen-mediated stimulation of the pleural cavity of rats passively sensitised with a single injection of IgE anti-DNP resulted in mast cell...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000049530
更新日期:2001-12-01 00:00:00
abstract::IL-3-dependent, murine mast cell lines derived from embryonic yolk sac precursors display a tumoricidal activity that is blocked by antibodies against tumor necrosis factor-alpha, indicating that this cytokine is the major mediator involved in the cytotoxic activity of the cultured mast cell lines. Further, cholera to...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236179
更新日期:1992-01-01 00:00:00
abstract::In the present study, we investigated the role of resident peritoneal cells as well as the mediators involved in the eosinophil migration induced by large volumes of physiological saline. Two consecutive intraperitoneal injections of saline given 48 h apart, induced a selective recruitment of eosinophils into the cavi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236687
更新日期:1994-08-01 00:00:00
abstract:BACKGROUND:The high-affinity IgE receptor, FcepsilonRI, is unresponsive on mast cells and basophils from people in several populations through an unknown mechanism. Similarly, FcepsilonRI-positive basophils from 'nonreleasers' are IgE-unresponsive and are deficient in the tyrosine kinase Syk. OBJECTIVE:To test the hyp...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000087355
更新日期:2005-09-01 00:00:00
abstract::Amino acid sequencing of the major mite allergen Der fII purified from mite body extract revealed that it is a mixture of at least two variants. Substitutions were found only at positions in which amino acid variations were predicted from the nucleotide sequences of three cloned cDNAs. When cDNAs corresponding to the ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236804
更新日期:1994-09-01 00:00:00
abstract::In all mammalian species investigated so far, mast cells and basophils are the only cells that synthesize histamine and express plasma membrane receptors that bind IgE with high affinity (Fc epsilonRI). Human basophils and mast cells derive from distinct precursors that originate in the bone marrow and fetal liver and...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000237670
更新日期:1997-11-01 00:00:00
abstract:BACKGROUND:Th17 is a subset of T-helper lymphocytes that produce proinflammatory cytokines, mainly IL-17. Serum IL-17 is increased in allergic patients and relates to clinical severity. Recently, it has been reported that CD161 is a highly upregulated gene in Th17 clones and all IL-17-producing cells are contained in C...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000330903
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:The population of large parts of Africa, South America and travellers to these areas are at risk of yellow fever (YF) with a 50% mortality risk. Yellow fever vaccine (YFV) propagated in hens' eggs confers protection in 95% of the vaccinated. The rate of anaphylaxis for YFV ranges from 0.42 to 1.8/100,000 dos...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000346350
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The hemagglutinin (HA) of influenza viruses is one of the major targets of the humoral response. The role of serum antibody to HA in the protection against infection has been demonstrated by long-standing observation. In previous studies, we suggested that an epitope vaccine might be a new strategy against t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053869
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Studies have shown that serum total immunoglobulin E (IgE) levels are higher in asthmatics. However, the role of the serum total IgE level, independently from atopy, in adult asthma is not understood. We studied the associations between serum total IgE, the number of sensitizations and the sum of specific Ig...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000342464
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Cat allergens are ubiquitous because the clothing of cat owners constitutes an important source of distribution of Fel d 1 in cat-free environments. Since Fel d 1 can adhere to a variety of surfaces, we sought to verify if human hair belonging to individuals with or without a cat at home might represent a re...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000085793
更新日期:2005-06-01 00:00:00
abstract:BACKGROUND:Lactation is associated with an inhibited hypothalamic-pituitary-adrenal axis response to physical and psychological stress in women and female rats. However, suckling also improved mood and calmness in nonatopic lactating women. Relaxation by humor reduced allergen-induced skin wheal responses, while variou...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000074906
更新日期:2003-12-01 00:00:00
abstract::We report a clinical study comparing the skin test reactivity to recombinant Aspergillus fumigatus allergen 1 (rAsp fI/a) in patients with atopic dermatitis and A. fumigatus sensitisation (n = 15), A. fumigatus-allergic patients with asthma (n = 10) and healthy control subjects (n = 10). All patients sensitised to A. ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237123
更新日期:1995-09-01 00:00:00
abstract::Many different mediators have been implicated in allergic responses and allergic diseases of the respiratory tract. The influence of several allergic inflammatory mediators on the ciliary activity has been well studied. However, ciliary responsiveness to platelet-activating factor (PAF) is yet to be established conclu...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236681
更新日期:1994-07-01 00:00:00
abstract::Platelet-activating factor (PAF)-induced augmentation of spontaneous differentiation into eosinophil-lineage cells from hematopoietic precursor cells was found among human umbilical cord blood mononuclear cells (CBMCs). This ability of PAF to augment eosinophil production was significantly (p < 0.01) inhibited by the ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236586
更新日期:1993-01-01 00:00:00