Abstract:
BACKGROUND:The hemagglutinin (HA) of influenza viruses is one of the major targets of the humoral response. The role of serum antibody to HA in the protection against infection has been demonstrated by long-standing observation. In previous studies, we suggested that an epitope vaccine might be a new strategy against the virus. METHODS:HA sequences of 491 H3 subtype strains from the influenza sequence database were compared and analyzed. To acquire information on the immunogenicity of the F3 epitope, F3-epitope-specific antibody levels in 81 patient sera infected with influenza virus were tested by ELISA. Based on the theory of the epitope vaccine, we designed an epitope peptide F3 (C-KAYSNCYPYDVPDY-G-KAYSNCYPYDVPDY), which contains the repeated F3 epitope KAYSNCYPYDVPDY (aa92-105) on HA (H3N2). The specificity and the titer of the antibodies induced by the epitope vaccine were determined by ELISA. The neutralizing activities of these anti-F3 antibodies were shown by inhibiting influenza virus infection of MDCK cells. RESULTS AND CONCLUSION:Comparison of HA sequences of 491 H3 subtype strains indicates that this epitope is highly conservative. Analysis of the sera from influenza virus-infected patients revealed a very low level of F3 epitope-specific antibodies, suggesting the poor immunogenicity of the F3 epitope on influenza virus. The epitope vaccine based on the F3 epitope induced high levels of F3 epitope-specific antibodies recognizing the epitope peptide F3 (antibody titer in antisera up to 1:25,600). Besides, the antisera could also recognize the natural HA in Western blotting. Interestingly, these antisera induced by the epitope vaccine could inhibit infection of MDCK cells by influenza virus (strain A/Wuhan/359/95) in the neutralization assay. These results suggest that the epitope vaccine can intensively increase the immunogenicity of neutralizing epitopes and may provide a new way to develop an effective vaccine against influenza virus.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Lu Y,Ding J,Liu W,Chen YHdoi
10.1159/000053869keywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
245-50issue
3eissn
1018-2438issn
1423-0097pii
53869journal_volume
127pub_type
杂志文章abstract:BACKGROUND:Patients with chronic urticaria (CU) in the UK could be referred to health care professionals (HCPs) with diverse specialties using different guidelines. The aims of the present study were to determine which CU guidelines HCPs in the UK use, which tests they use for the diagnosis of CU, and how they manage C...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000430442
更新日期:2015-01-01 00:00:00
abstract:AIM:We evaluated the effect of the 45-kD protein of Trichinella spiralis (gp45), purified by affinity chromatography, on random migration and chemotaxis, the oxidative metabolism of human neutrophils and on the CD11b upregulation induced by formyl-methionyl-leucyl-phenylalanine (f-MLP). METHODS:Donor neutrophils incub...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024359
更新日期:2000-05-01 00:00:00
abstract:BACKGROUND:In a subgroup of patients with atopic eczema (AE), aeroallergens are relevant eliciting factors. The atopy patch test (APT) was proposed as inflammation model for AE. OBJECTIVE:It was the aim of this study to investigate the effect of pretreatment with 1% pimecrolimus cream (Elidel) on the APT and skin pric...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000093249
更新日期:2006-01-01 00:00:00
abstract::Interaction between vascular cell adhesion molecule-1 (VCAM-1) on endothelial cells and CD49d molecules on eosinophils is thought to mediate the selective infiltration of eosinophils into inflamed tissues in allergic disease. IL-4 and TNF-alpha are associated with allergic conditions, and they have been shown to selec...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237357
更新日期:1996-10-01 00:00:00
abstract:BACKGROUND:Reports on oral immunotherapy (OIT) for anaphylactic food allergy are lacking. We investigated the efficacy and safety of peanut OIT for anaphylactic patients. METHODS:We enrolled 22 peanut anaphylactic patients who underwent OIT between 2011 and 2013, all of whom demonstrated anaphylaxis during a baseline ...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000486310
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Volatile monoisocyanates are formed through thermal degradation when products containing polyurethane are heated. Repeated exposure to diisocyanates, such as toluene diisocyanate (TDI) are a well-known cause of occupational asthma. However, although monoisocyanates are abundant in occupational settings, ther...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000222675
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Sensitivity to the mite Blomia tropicalis is related to asthma in tropical climates, but correlates of sensitivity to B. tropicalis and its relationship to Dermatophagoides pteronyssinus sensitivity have not been widely examined in families with asthma. The main objective of this study was to determine preva...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000070927
更新日期:2003-06-01 00:00:00
abstract::Contact sensitivity to para-phenylenediamine (PPD) is a frequent delayed-type hypersensitivity resulting in contact dermatitis. The aim of the present study, conducted in 16 patients allergic to PPD (as assessed by a positive patch test), was to get better insight into the mechanism of T-cell activation in PPD contact...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236573
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Intravenous immunoglobulin (IVIG) is the mainstay therapy in human immune deficiency states characterized by qualitative and quantitative reductions in B cells. In addition, however, there is widespread use of IVIG in a number of other areas, including neuroimmunologic, infectious, dermatologic, hematologic,...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000093204
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Epidemiological data on food allergy are scarce in African countries. We studied the prevalence of food sensitization in Ghanaian schoolchildren. METHODS:Children (5-16 years; n = 1,714) from 9 Ghanaian schools were given parental consent to participate in the study. Adverse reactions and food consumption w...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000318704
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Chronic urticaria is associated with an immune dysregulation usually mediated by T lymphocytes. Recently, Th17 and Tc17 have been implicated in autoimmune diseases; however, their role in urticaria is not clear yet. METHODS:For the study we recruited 20 patients [10 of them had autoreactive chronic spontane...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000345401
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The cervical sympathetic trunk-submandibular gland (CST-SMG) axis plays an important role in inflammation. An immunoregulatory heptapeptide, submandibular gland peptide T (SGP-T), was isolated from submandibular glands, and several of its biological activities have been identified. RESULTS:SGP-T reduced sho...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237594
更新日期:1997-05-01 00:00:00
abstract::We report a clinical study comparing the skin test reactivity to recombinant Aspergillus fumigatus allergen 1 (rAsp fI/a) in patients with atopic dermatitis and A. fumigatus sensitisation (n = 15), A. fumigatus-allergic patients with asthma (n = 10) and healthy control subjects (n = 10). All patients sensitised to A. ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237123
更新日期:1995-09-01 00:00:00
abstract::Owing to the proposed role of Fc epsilon RII/CD23 in allergic diseases, we analyzed the expression of this receptor on peripheral blood lymphocytes (pan-B, pan-T and CD4+ or CD8+ T cells) and its autoproteolytic product sCD23 in serum. This was done in 10 asthmatic children allergic to Dermatophagoides pteronyssinus (...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236520
更新日期:1993-01-01 00:00:00
abstract::Ovalbumin (OVA)-pullulan conjugates were made using four different conjugation methods and eight different molecular weights of pullulan ranging from 4,200 to 600,000. Pretreatment of mice by the administration of conjugates made by using cyanulic chloride or cyanogen bromide and pullulan of molecular weight 40,000 or...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236536
更新日期:1993-01-01 00:00:00
abstract::Preferential eosinophil accumulation is characteristic of airway inflammation in asthma. Although little is known about its mechanism, the effect of a sialyl Lewis X analog on airway eosinophilia was examined in a guinea-pig model of asthma. Guinea-pigs were sensitized by repeated inhalation of ovalbumin. After a sing...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237412
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:Shrimp sensitization is common in the general population, but the presence of symptoms is only moderately related to sensitization. A point still at issue is which in vivo and/or in vitro tests (food challenge, component-resolved diagnosis, house dust mite [HDM] sensitization) can help in distinguishing shri...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000481985
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Orange allergens are virtually unknown, in spite of the large consumption of this fruit. Germin-like proteins, together with vicilins and legumins, form the cupin superfamily of plant proteins, which includes many seed allergens. METHODS:Twenty-nine patients with allergy to oranges were studied. A major IgE...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000090384
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Human leukocyte antigen G (HLA-G) is a nonclassical major histocompatibility complex class I gene. HLA-G stimulates Th2 cytokine secretion by peripheral blood mononuclear cells. The role of soluble HLA-G (sHLA-G) in bronchial asthma is incompletely understood and the plasma level of sHLA-G in asthmatic child...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000095290
更新日期:2006-01-01 00:00:00
abstract::Two cDNA clones named P9* and P1* of 794 and 631 bp, respectively, were isolated from a lambda ZAP cDNA expression library using Parietaria judaica (Pj) pollen-specific IgE antibodies from a pool of sera (n = 23) of patients allergic to Pj. Sequence analysis showed open reading frames of 176 and 138 amino acids. Both ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237479
更新日期:1997-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Nickel sulphate stimulates the proliferation of lymphocytes in nickel-allergic subjects. However, nickel-induced stimulation of lymphocytes from control persons without clinical symptoms of nickel allergy has also been reported. The aim of the present study was to correlate T cell activity, mea...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024437
更新日期:2000-10-01 00:00:00
abstract:BACKGROUND:Airway closure is frequently observed in human asthma. However, limited information exists on the factors that cause this condition. In this study, an allergic cynomolgus monkey model was used to characterize the condition of airway closure and assess the contribution of histamine H1 receptors to this respon...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000084611
更新日期:2005-05-01 00:00:00
abstract:BACKGROUND:Elicitation of contact hypersensitivity requires antigen-specific immunoglobulin M (IgM) antibodies that trigger recruitment of effector T cells to the local tissue. These antigen-specific IgM antibodies are produced by B-1-like 'initiator B cells'. In this study, we compared susceptibility to hapten-induced...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000350367
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Clustered severe adverse reactions to immunotherapy with olive pollen extracts have been occasionally reported in areas where olive trees are extensively grown. Allergic patients from these areas, in addition to the major olive pollen allergen Ole e 1, frequently recognize a recently described allergen, Ole ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000092532
更新日期:2006-01-01 00:00:00
abstract::To determine the variation of antigenic water soluble proteins in white birch (Betula pendula) pollen, extracts of pollen from different sides of individual trees were analyzed by isoelectric focusing (IEF), crossed immunoelectrophoresis, and crossed radioimmunoelectrophoresis. IgE-antibody-binding patterns were also ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237184
更新日期:1995-12-01 00:00:00
abstract:BACKGROUND:Recombinant allergens are preferred over natural allergen extracts in measuring antibodies. We tested the use of recombinant variants of the major mouse allergen Mus m 1 in detection of mouse-specific antibodies in sera of laboratory animal workers and children. METHODS:Six recombinant major urinary protein...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000106318
更新日期:2007-01-01 00:00:00
abstract::The modulatory role of hormones in the regulation of the immune response has been well documented. Here, we present some thoughts on how sex hormones affect immunity, particularly at mucosal sites. We further discuss the possible pitfalls and difficulties associated with analysis of the data. ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000236631
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:It is important to understand that allergic rhinitis, whether seasonal or perennial, may be difficult to distinguish clinically from the nonallergic forms of rhinitis. OBJECTIVE:This study aimed to investigate the presence of local allergic rhinitis (LAR) in children who have allergic rhinitis symptoms in t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000447635
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:The digestive system of Dermatophagoides farinae remains poorly documented. METHODS:In this study, a three-dimensional (3-D) model of the alimentary canal of D. farinae was built for the first time based on hematoxylin-eosin staining, 76 cross-sections of the adult mite and 3-D reconstruction technology. R...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000115890
更新日期:2008-01-01 00:00:00
abstract:INTRODUCTION:Obesity-associated asthma is characterized by type 2-low airway inflammation. We previously showed that EM900, which is a 12-membered nonantibiotic macrolide, suppressed airway inflammation in a mouse model of asthma exacerbation. The aim of this study was to clarify the effects of EM900 in obesity-associa...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000508709
更新日期:2020-01-01 00:00:00