Detection of antigens reactive to IgE and IgA during wheat seed maturation and in different wheat cultivars.

Abstract:

BACKGROUND:Dietary intake of wheat causes hypersensitivity reactions in patients suffering from IgE-mediated food allergy and coeliac disease. AIM:To study the expression of IgE- and IgA-reactive antigens during wheat seed maturation and in different wheat cultivars. METHODS:Summer wheat was grown in a glasshouse and seeds were harvested at defined maturation stages. Mature seeds were obtained from 13 different defined cultivars. Protein extracts were prepared from different maturation stages and cultivars with a standardized procedure based on seed weight and analysed by IgE and IgA immunoblotting using sera from clinically defined patients suffering from wheat allergy or coeliac disease. RESULTS:With a few exceptions the expression of IgE- and IgA-reactive wheat antigens increased during wheat seed maturation. Wheat cultivars could be identified in which the expression of certain IgE- and IgA-reactive components was strongly reduced or not detectable. CONCLUSIONS:The expression of IgE- and IgA-reactive antigens depends on wheat seed maturation and varies in different wheat cultivars.

authors

Constantin C,Touraev A,Heberle-Bors E,Quirce S,Huber WD,Valenta R

doi

10.1159/000199713

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

181-7

issue

3

eissn

1018-2438

issn

1423-0097

pii

000199713

journal_volume

149

pub_type

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