Comparison of the antigenic and allergenic properties of three types of bovine epithelial material.

Abstract:

:The antigenic and allergenic characteristics of three bovine epithelial extracts, dander, skin scrapings and whole skin, were compared using IgE-ELISA inhibition and SDS-PAGE with immunoblotting. Cow dander extract was shown to contain more allergenic activity than skin scrapings or whole skin extracts which were needed in about three times higher amounts than cow dander extract to induce the same degree of inhibition in ELISA. Skin scrapings and whole skin extracts contained more high-molecular weight components than dander extract. These components were at least partly serum-derived and reacted often with the IgG but not with the IgE of both the cow-asthmatics and their control subjects. The antigenic characteristics of the low-molecular weight components as well as the allergenic qualities of these three epithelial preparations were generally similar. Using the sera of 49 cow-asthmatic farmers, two major allergens were detected at 20 and 22 kD in all three extracts. Our results show that the highest amount of allergenic material and all the essential allergens are present in cow dander extract. In addition, the normally non-allergenic high molecular weight components are detected in low concentrations in dander extract. Therefore it is concluded that cow dander extract is the best alternative for allergen purification and allergen extract preparation.

authors

Ylönen J,Mäntyjärvi R,Taivainen A,Virtanen T

doi

10.1159/000236343

keywords:

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

112-7

issue

1

eissn

1018-2438

issn

1423-0097

journal_volume

99

pub_type

杂志文章
  • Isolation and preparation of antisera to ovine IgE.

    abstract::Elevated levels of ovine reaginic antibody were induced by immunization with Ascaris suum antigens. The PCA activity of this antibody persisted in the skin of sheep for 20 days and was abolished by heating to 56 degrees C, suggesting that it was immunoglobulin E. Attempts to isolate IgE from this hyperimmune serum by ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000236479

    authors: Yilmaz H,Roe JM,Morgan KL

    更新日期:1993-01-01 00:00:00

  • Analysis of gamma4 germline transcription in human B cells.

    abstract:BACKGROUND:Allergic reactions occur rarely in patients with chronic helminth infections, although FcepsilonRI-bearing cells are sensitized with anti-parasite IgE and are exposed to antigen. The inhibition of allergic reactivity is due to 'blocking antibodies', predominantly IgG4. IgG4 antibodies represent 50-95% of ant...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000024099

    authors: Agresti A,Vercelli D

    更新日期:1999-02-01 00:00:00

  • Hypersensitivity Reactions to Nonsteroidal Anti-Inflammatory Drugs among Adults: Clinical Features and Risk Factors for Diagnosis Confirmation.

    abstract:BACKGROUND:Nonsteroidal anti-inflammatory drugs (NSAIDS) are among the most common causes of drug hypersensitivity (HS) reactions. The diagnosis is based on a careful clinical history, and provocation tests are considered the gold standard for diagnosis. Skin tests have some value to study reactions to pyrazolones. Lab...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000452627

    authors: Rebelo Gomes E,Geraldes L,Gaspar Â,Malheiro D,Cadinha S,Abreu C,Chambel M,Almeida E,Faria E,Portuguese Society of Allergology and Clinical Immunology (SPAIC) Drug Allergy Interest Group.

    更新日期:2016-01-01 00:00:00

  • Intratracheal application of Sephadex in rats leads to massive pulmonary eosinophilia without bronchial hyperreactivity to acetylcholine.

    abstract::Fourteen Brown-Norway rats were pretreated with physiological saline (n = 7) or 500 micrograms Sephadex (n = 7) intratracheally. 24 h later, a bronchial provocation test was performed under pentobarbital anaesthesia using increasing doses of acetylcholine aerosol and the degree of bronchospasm was measured using a mod...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000236196

    authors: Kubin R,Deschl U,Linssen M,Wilhelms OH

    更新日期:1992-01-01 00:00:00

  • Dissociation of allergenic and immunogenic functions in contact sensitivity to para-phenylenediamine.

    abstract::Contact sensitivity to para-phenylenediamine (PPD) is a frequent delayed-type hypersensitivity resulting in contact dermatitis. The aim of the present study, conducted in 16 patients allergic to PPD (as assessed by a positive patch test), was to get better insight into the mechanism of T-cell activation in PPD contact...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000236573

    authors: Krasteva M,Nicolas JF,Chabeau G,Garrigue JL,Bour H,Thivolet J,Schmitt D

    更新日期:1993-01-01 00:00:00

  • Detection of antibodies against streptococcal peptidoglycan and the peptide subunit (synthetic tetra-D-alanyl-bovine serum albumin complex) in rheumatic-diseases.

    abstract::Serum antibodies reactive with streptococcal cell wall peptidoglycan (PG) and its peptide subunit (synthetic tetra-D-alanine) were measured by enzyme-linked immunosorbent assay (ELISA) in patients with rheumatoid arthritis (RA), juvenile rheumatoid arthritis (JRA), osteoarthritis and acute rheumatic fever (RF) compare...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000236137

    authors: Todome Y,Ohkuni H,Mizuse M,Furuya M,Fujikawa S,Tanaka S,Watanabe N,Fujii K,Zabriskie JB

    更新日期:1992-01-01 00:00:00

  • A functional study on CysLT(1) receptors in human eosinophils.

    abstract:BACKGROUND:The cysteinyl leukotrienes (CysLTs) mediate their biological actions through two receptors: CysLT(1) receptor and CysLT(2) receptor. OBJECTIVE:This study was undertaken to examine the direct effects of CysLTs on eosinophils, such as chemotaxis and degranulation, focusing on CysLT(1). METHODS:Eosinophils we...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000065175

    authors: Ohshima N,Nagase H,Koshino T,Miyamasu M,Yamaguchi M,Hirai K,Yamamoto K,Fujisawa T,Nakagawa N,Kishikawa K,Morita Y

    更新日期:2002-09-01 00:00:00

  • Changes in plasma TARC levels during Japanese cedar pollen season and relationships with symptom development.

    abstract:BACKGROUND:Japanese cedar pollinosis (JCPsis) is an immunoglobulin E-mediated type I allergy caused by exposure to Japanese cedar pollen (JCP). Blood thymus and activation-regulated chemokine (TARC) levels are well known as an objective parameter for disease severity for several allergic disorders. The present study ai...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000103223

    authors: Xiao JZ,Kondo S,Takahashi N,Odamaki T,Iwabuchi N,Miyaji K,Iwatsuki K,Enomoto T

    更新日期:2007-01-01 00:00:00

  • Elevated numbers of cells producing interleukin-5 and interleukin-10 in a boy with Kimura disease.

    abstract::Kimura disease is a rare disorder of unknown etiology, characterized by the presence of benign subcutaneous granuloma, marked peripheral blood eosinophilia and elevation of the immunglobulin E (IgE) serum level. Here, we present a case of a 12-year-old boy with Kimura disease who had a history of repeated severe influ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000337777

    authors: Hosoki K,Hirayama M,Kephart GM,Kita H,Nagao M,Uchizono H,Toyoda H,Senba Y,Imai Y,Komada Y,Ihara T,Fujisawa T

    更新日期:2012-01-01 00:00:00

  • Adjuvant effect of pertussis toxin on the production of anti-ovalbumin IgE in mice and lack of direct correlation between PCA and ELISA.

    abstract::In studies designed to optimize the production and detection of anti-ovalbumin (anti-Oa) IgE in Ham/ICR mice, a range of doses of both Oa and pertussis toxin as the IgE adjuvant was explored. As determined by 48-hour passive cutaneous anaphylaxis (PCA) tests, the highest titre of anti-Oa IgE was obtained in a three-in...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000236770

    authors: Lindsay DS,Parton R,Wardlaw AC

    更新日期:1994-11-01 00:00:00

  • Ultrastructural observation of adherence of eosinophils to their surroundings.

    abstract::This study concerned the way in which eosinophils adhere to their surroundings. Many fibrils were seen to adhere to the cell membrane of eosinophils migrating into the submucosa. Those eosinophils migrating into the epithelial layer adhered to the epithelial cells in 2 different ways: through the thin filaments bridgi...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000237199

    authors: Watanabe K

    更新日期:1995-01-01 00:00:00

  • Cloning and molecular characterization of a cDNA from Blomia tropicalis homologous to dust mite group 3 allergens (trypsin-like proteases).

    abstract:BACKGROUND:Exposure to the house dust mite Blomia tropicalis is increasingly being implicated as a major risk factor for asthma exacerbations in sensitized individuals. The objective of this study is to clone and characterize B. Tropicalis allergens in order to better define their role in allergic asthma. METHODS:A la...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000068375

    authors: Flores I,Mora C,Rivera E,Donnelly R,Montealegre F

    更新日期:2003-01-01 00:00:00

  • T cells and chronic asthma.

    abstract::There is increasing evidence that the asthma process is 'driven' and maintained by persistence of a subset of chronically activated T memory cells, sensitized against allergenic, occupational or viral antigens which 'home' to the lung after antigen exposure or viral infection. In general, allergens induce a CD4 T help...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章,评审

    doi:10.1159/000024049

    authors: Kon OM,Kay AB

    更新日期:1999-02-01 00:00:00

  • Single Nucleotide Polymorphisms in Chemosensory Pathway Genes GNB3, TAS2R19, and TAS2R38 Are Associated with Chronic Rhinosinusitis.

    abstract:BACKGROUND:Chronic rhinosinusitis (CRS) is a multifaceted disease with a significant genetic component. The importance of taste receptor signaling has recently been highlighted in CRS; single nucleotide polymorphisms (SNPs) of bitter tastant-responsive G-protein-coupled receptors have been linked with CRS and with alte...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000499875

    authors: Purnell PR,Addicks BL,Zalzal HG,Shapiro S,Wen S,Ramadan HH,Setola V,Siderovski DP

    更新日期:2019-01-01 00:00:00

  • Inhibitory effects of human neutrophil functions by the 45-kD glycoprotein derived from the parasitic nematode Trichinella spiralis.

    abstract:AIM:We evaluated the effect of the 45-kD protein of Trichinella spiralis (gp45), purified by affinity chromatography, on random migration and chemotaxis, the oxidative metabolism of human neutrophils and on the CD11b upregulation induced by formyl-methionyl-leucyl-phenylalanine (f-MLP). METHODS:Donor neutrophils incub...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000024359

    authors: Bruschi F,Carulli G,Azzarà A,Homan W,Minnucci S,Rizzuti-Gullaci A,Sbrana S,Angiolini C

    更新日期:2000-05-01 00:00:00

  • Defective 'gut processing' of gliadin in mice with graft-versus-host enteropathy.

    abstract::The gut mucosa plays an important role in the induction of oral tolerance. Extending previous observations, we have here shown that serum containing gut-absorbed gliadin induces suppression of the specific systemic immune response in recipient mice parenterally immunized with gliadin. Graft-versus-host reaction (GvHR)...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000237230

    authors: Troncone R,Caputo N,Zibella A,Russo R,Rossi M,Gianfrani C,Stern M,Wieser H,Auricchio S

    更新日期:1996-01-01 00:00:00

  • Smoking attenuates increase in exhaled nitric oxide in atopic but not in nonatopic young adults with asthma.

    abstract:BACKGROUND:Measurement of fractional exhaled nitric oxide (FENO) is useful in assessing eosinophilic airway inflammation. Smoking may modify airway inflammation and reduce FENO levels, compromising the diagnostic value of FENO in smokers. How smoking influences FENO in atopic versus nonatopic asthmatics is unknown. The...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000283029

    authors: Rouhos A,Ekroos H,Karjalainen J,Sarna S,Haahtela T,Sovijärvi AR

    更新日期:2010-01-01 00:00:00

  • Interest of ImmunoCAP system to recombinant omega-5 gliadin for the diagnosis of exercise-induced wheat allergy.

    abstract:BACKGROUND:omega-5 gliadin is a major allergen in exercise-induced wheat allergy (EIWA), but it is also implicated in immediate-type reactions to wheat. An ImmunoCAP assay to measure omega-5 gliadin-specific IgE has become available. This study aimed to evaluate this new biological test in wheat allergy diagnosis and t...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000176309

    authors: Jacquenet S,Morisset M,Battais F,Denery-Papini S,Croizier A,Baudouin E,Bihain B,Moneret-Vautrin DA

    更新日期:2009-01-01 00:00:00

  • Hypersensitivity pneumonitis associated with mushroom worker's lung: an update on the clinical significance of the importation of exotic mushroom varieties.

    abstract::Hypersensitivity pneumonitis remains an important industrial disease in mushroom workers. It has a significant morbidity, and early diagnosis and removal from exposure to the antigen are critically important in its management. Recently, several new allergens have been described, particularly those from mushroom specie...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章,评审

    doi:10.1159/000083298

    authors: Moore JE,Convery RP,Millar BC,Rao JR,Elborn JS

    更新日期:2005-01-01 00:00:00

  • Suppressive effects of SP-A on ionomycin-induced IL-8 production and release by eosinophils.

    abstract::Recent studies have demonstrated that pulmonary surfactant protein (SP)-A plays a potential role in modifying inflammation and immune function. To see whether SP-A could modify IL-8 production and release by eosinophils stimulated with ionomycin, SP-A purified from surfactant recovered from patients with alveolar prot...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000053574

    authors: Cheng G,Ueda T,Nakajima H,Nakajima A,Kinjyo S,Motojima S,Fukuda T

    更新日期:1998-09-01 00:00:00

  • Differential effects of short-acting beta2-agonists on human granulocyte functions.

    abstract:BACKGROUND:beta2-Adrenergic agonists play a pivotal role in the management of bronchial asthma. Although the major effect of short-acting beta2-agonists on the airway is relaxation of smooth muscles, they may also have several effects on surrounding immunomodulatory cells. METHODS:We examined whether widely used short...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000089516

    authors: Yasui K,Kobayashi N,Yamazaki T,Agematsu K,Matsuzaki S,Nakata S,Baba A

    更新日期:2006-01-01 00:00:00

  • Vitamin C and E suppress mitogen-stimulated peripheral blood mononuclear cells in vitro.

    abstract:BACKGROUND/AIMS:The antioxidant properties of vitamin C and E are considered to be important for their anti-inflammatory activity. Recently, antioxidant resveratrol was found to suppress neopterin production and tryptophan degradation in mitogen-treated human peripheral blood mononuclear cells. METHODS:In this study, ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000096438

    authors: Winkler C,Schroecksnadel K,Schennach H,Fuchs D

    更新日期:2007-01-01 00:00:00

  • Identification of alpha-amylase inhibitor as a major allergen of wheat flour.

    abstract::We isolated a major wheat flour allergen with a molecular weight of 15-kD. The 15-kD allergen was purified from an extract of wheat flour by ammonium sulfate precipitation followed by gel filtration. Using the immunoblot technique, IgE, specifically directed towards the single isolated allergen, was detectable in 50% ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000236726

    authors: Fränken J,Stephan U,Meyer HE,König W

    更新日期:1994-01-01 00:00:00

  • The Gut Microbiome of Adults with Allergic Rhinitis Is Characterised by Reduced Diversity and an Altered Abundance of Key Microbial Taxa Compared to Controls.

    abstract:INTRODUCTION:Unique gut microbial colonisation patterns are associated with the onset of allergic disease in infants; however, there is insufficient evidence to determine if aberrant microbial composition patterns persist in adult allergic rhinitis (AR) sufferers. OBJECTIVE:To compare the gut microbiome composition be...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000510536

    authors: Watts AM,West NP,Zhang P,Smith PK,Cripps AW,Cox AJ

    更新日期:2021-01-01 00:00:00

  • CD40 ligand/CD40 deficiency.

    abstract::CD40 is a surface antigen expressed on B cells. The CD40 ligand (CD40L) is expressed on activated T cells. Interaction between CD40 and CD40L is critical for proliferation and isotype switching in the context of a response to a T-cell-dependent antigen. Patients with X-linked hyper-IgM syndrome (HIGMX-1) in their CD40...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章,评审

    doi:10.1159/000236923

    authors: Castigli E,Fuleihan R,Ramesh N,Tsitsikov E,Tsytsykova A,Geha RS

    更新日期:1995-05-01 00:00:00

  • Chlorhexidine Anaphylaxis Masquerading as Septic Shock.

    abstract::Chlorhexidine is a commonly used antiseptic and disinfectant in the health-care setting. Its usage has increased in recent years with intensive campaigns and infection control guidelines to combat hospital-acquired infections. As a result, patients and health-care workers (HCW) are exposed to increasing chlorhexidine ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000431358

    authors: Hong CC,Wang SM,Nather A,Tan JH,Tay SH,Poon KH

    更新日期:2015-01-01 00:00:00

  • Immunochemical characterization of rapid and slowly released allergens from the pollen of Parthenium hysterophorus.

    abstract::Allergens from the pollen of Parthenium hysterophorus (American feverfew), responsible for high incidence of allergic rhinitis, were found by immunoprint analysis to be localized on the surface of the pollen grains. The allergens were released very rapidly when extracted in vitro. The allergenic activity of the rapid ...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000237099

    authors: Gupta N,Sriramarao P,Kori R,Rao PV

    更新日期:1995-08-01 00:00:00

  • Sex Differences in Adults with Insect Venom Allergy regarding Psychological Distress, Attitudes toward the Emergency Medication, and Factual Knowledge about the Disease.

    abstract:BACKGROUND:Hymenoptera venom allergy (HVA) has a prevalence of 3% in adults. Although patients have no ongoing symptoms, they often suffer from an impairment of their psychological well-being and quality of life. OBJECTIVE:The aim of this study was to analyze sex-specific differences regarding the psychological burden...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000507625

    authors: Schoeben LS,Bubak C,Schaarschmidt ML,Schmieder A

    更新日期:2020-01-01 00:00:00

  • Effectiveness and Safety of Omalizumab in Patients with Allergic Bronchopulmonary Aspergillosis Complicated by Chronic Bacterial Infection in the Airways.

    abstract:BACKGROUND:Allergic bronchopulmonary aspergillosis (ABPA) develops in the presence of predisposing conditions such as asthma and cystic fibrosis. Even ABPA accompanied by asthma is often complicated by chronic Pseudomonas aeruginosa or nontuberculous mycobacterial infection of the lower respiratory tract, rendering tre...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000507216

    authors: Tomomatsu K,Oguma T,Baba T,Toyoshima M,Komase Y,Taniguchi M,Asano K,Japan ABPM Research Program.

    更新日期:2020-01-01 00:00:00

  • Sera from patients with multiple drug allergy syndrome contain circulating histamine-releasing factors.

    abstract:BACKGROUND:A subset of drug-intolerant patients show a marked propensity to react to several chemically unrelated antibacterial drugs. This condition is termed multiple drug allergy syndrome (MDAS). The pathogenesis of MDAS is still unclear. A possible mechanism is that a nonspecific patient-related factor leading to d...

    journal_title:International archives of allergy and immunology

    pub_type: 杂志文章

    doi:10.1159/000071486

    authors: Asero R,Tedeschi A,Lorini M,Caldironi G,Barocci F

    更新日期:2003-07-01 00:00:00