Abstract:
BACKGROUND:Exposure to the house dust mite Blomia tropicalis is increasingly being implicated as a major risk factor for asthma exacerbations in sensitized individuals. The objective of this study is to clone and characterize B. Tropicalis allergens in order to better define their role in allergic asthma. METHODS:A lambda gt22A cDNA library was constructed from B. Tropicalis mRNA and screened using specific DNA probes. A full-length cDNA (Bt2-3) was isolated, subcloned, and sequenced. RESULTS:Sequence analysis showed that clone Bt2-3 encodes the full-length serine protease preproenzyme from B. Tropicalis. The predicted Bt2-3 protein consists of a 15-amino-acid signal peptide, a 20-amino-acid propeptide and a mature protein of 231 amino residues. BLAST analysis of the deduced amino acid sequence showed high homology (48-54%) between clone Bt2-3 and serine proteases (group 3 allergens) from domestic dust mites. Both the serine active site (DACQGDSGGPVA; amino acids 214-225) and the histidine active site (LTAAHC; amino acids 71-76) of serine proteases were highly conserved. The estimated molecular weight of the preproenzyme is 27.5 kD and its theoretical pI is 8.7. The mature protein contains a putative tyrosine kinase phosphorylation site (amino acids 24-31) and several N-myristoylation sites. Sequence alignment shows that the serine protease active sites are highly conserved among the clinically important mite species, including B. Tropicalis. CONCLUSION:We report the cloning and molecular characterization of a B. Tropicalis cDNA clone encoding a trypsin-like protease, with a possible major role as an allergen. Expression and further characterization of the recombinant product will help determine the role of proteolytic enzymes in the pathophysiology of allergic asthma.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Flores I,Mora C,Rivera E,Donnelly R,Montealegre Fdoi
10.1159/000068375keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
12-6issue
1eissn
1018-2438issn
1423-0097pii
68375journal_volume
130pub_type
杂志文章abstract:BACKGROUND:Interleukin (IL)-33, a new member of the IL-1 cytokine family, has been recognized as a key cytokine that enhances T helper 2-balanced immune regulation through its receptor ST2; however, the function and relationship of the IL-33 and ST2 pathways in bronchial asthma are still unclear. We investigated the ce...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000327259
更新日期:2011-01-01 00:00:00
abstract::A 20mer peptide representing the N-terminus of a major allergen, Alt a I, of Alternaria alternata was synthesized and examined for its antibody binding and antibody induction activities. Alt a I peptide-BSA conjugate reacted with both human IgE and rabbit anti-Alt a I IgG in ELISA, albeit the binding of the peptide to...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237161
更新日期:1995-11-01 00:00:00
abstract:BACKGROUND:Cry j 1 and Cry j 2 are thought to be the major allergens of Japanese cedar pollen. HLA class II types capable of presenting T-cell epitopes in both allergens and their role in induction of T-cell subsets are not well known. METHODS:CD4+ T (Th)-cell clones (TCCs) specific to either Cry j 1 or Cry j 2 were g...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024194
更新日期:1999-07-01 00:00:00
abstract:BACKGROUND:Numerous strategies have been proposed for the treatment of peanut allergies, but despite the steady advancement in our understanding of atopic immune responses and the increasing number of deaths each year from peanut anaphylaxis, there is still no safe, effective, specific therapy for the peanut-sensitive ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053672
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:L-selectin (CD62L) and Mac-1 (CD11b/CD18) play a crucial role in the infiltration of eosinophils. However, changes in CD62L and CD11b expression on eosinophils after stimulation with cytokines were little studied. METHODS:Eosinophils in peripheral blood of healthy volunteers were purified and cultured with ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053590
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Th2 cytokines play pivotal roles in allergic inflammation, including eosinophilia, and their actions are antagonized by Th1 cytokines, conferring them therapeutic potential. METHODS:In this study, we examined the ability of a number of cytokines to suppress the activation of eosinophils that function as eff...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000083957
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND:Over the past years, dust of green coffee beans has become known to be a relevant cause for occupational type I allergies. Up to now, allergy diagnostics is based on native green coffee bean extract which exhibits insufficient specificity due to interfering substances as well as batch-to-batch variations. No...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000337461
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:It has been shown that antigen presentation by resting B cells can induce tolerance to intravenously administered protein antigens, but the role of resting B cells in the induction of oral tolerance is unclear. METHODS:Mice continuously treated since birth with rabbit anti-mouse IgM serum for 5 weeks were d...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024394
更新日期:2000-07-01 00:00:00
abstract:BACKGROUND:Allergies and asthma exhibit a growing health problem in the world. Sparse data are available about the prevalence of allergic sensitization in Estonia and Eastern Europe as well. We studied the prevalence of allergic sensitization, the associations between respiratory symptoms and allergic sensitization, an...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000097027
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Exposure and sensitization to pet allergens are associated with allergic asthma in children. Conflicting data have emerged regarding the potential benefit of air cleaners with respect to a reduction of indoor pet allergens and bronchial hyperresponsiveness (BHR). METHODS:In a randomized controlled trial 36 ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000151502
更新日期:2009-01-01 00:00:00
abstract:AIM:We evaluated the effect of the 45-kD protein of Trichinella spiralis (gp45), purified by affinity chromatography, on random migration and chemotaxis, the oxidative metabolism of human neutrophils and on the CD11b upregulation induced by formyl-methionyl-leucyl-phenylalanine (f-MLP). METHODS:Donor neutrophils incub...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024359
更新日期:2000-05-01 00:00:00
abstract:BACKGROUND:Common variable immunodeficiency (CVID) is the most common primary antibody deficiency syndrome in humans, but it remains a diagnosis of exclusion in most cases. Several genetically defined primary immunodeficiencies mimic CVID. Among them is the X-linked lymphoproliferative syndrome (XLP) which was shown to...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000135702
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND AND AIM:Although still controversial, drug provocation tests (DPTs) are considered by many as the gold standard for diagnosis of drug hypersensitivity. No studies have addressed the opinion of patients submitted to DPT. We aimed to determine patient satisfaction with diagnostic DPT and their perception of it...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,多中心研究
doi:10.1159/000323898
更新日期:2011-01-01 00:00:00
abstract::A case of anaphylaxis following a bite from a Siberian hamster (SH; Phodopus sungorus) is described. Skin prick tests with hair, urine and salivary gland extracts from SH were positive, while the tests were negative for hair extracts from other rodents. IgE immunoblotting with the patient serum revealed 3 IgE-binding ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000324956
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Penicillium chrysogenum (Penicillium notatum) is a prevalent airborne Penicillium species. A 34-kD major IgE-reacting component from P. chrysogenum has been identified as an alkaline serine protease (Pen ch 13, also known as Pen n 13 before) by immunoblot and N-terminal amino acid sequence analysis. METHODS...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000048165
更新日期:2002-01-01 00:00:00
abstract::The case of a 46-year-old man with a chronic cough with sputum eosinophilia (atopic cough) caused by Trichosporon cutaneum serotype II (Trichosporon asahii) is reported. The diagnosis was made with the inhalation challenge test with T. asahii antigen. He was admitted for the diagnosis and treatment of a severe nonprod...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000023940
更新日期:1998-06-01 00:00:00
abstract:BACKGROUND:The implementation of allergen immunotherapy (AIT) requires extensive knowledge of allergen distribution in the region to identify high-risk regions for AIT utilization. However, the geographical distribution patterns of the major Dermatophagoides allergens in China remain unclear despite the increasing prev...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000499409
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:The cysteinyl leukotrienes (CysLTs) mediate their biological actions through two receptors: CysLT(1) receptor and CysLT(2) receptor. OBJECTIVE:This study was undertaken to examine the direct effects of CysLTs on eosinophils, such as chemotaxis and degranulation, focusing on CysLT(1). METHODS:Eosinophils we...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000065175
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND:The association between staphylococcal enterotoxins and atopic dermatitis (AD) is well characterized. We aim to evaluate the association between sensitization to staphylococcal enterotoxin A (SEA) and/or B (SEB) and the development of allergic airway disease. METHODS:Two hundred and seventy-four patients we...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000088868
更新日期:2005-12-01 00:00:00
abstract:BACKGROUND:The population of large parts of Africa, South America and travellers to these areas are at risk of yellow fever (YF) with a 50% mortality risk. Yellow fever vaccine (YFV) propagated in hens' eggs confers protection in 95% of the vaccinated. The rate of anaphylaxis for YFV ranges from 0.42 to 1.8/100,000 dos...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000346350
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Exposure to Aspergillus fumigatus allergens results in the sensitization and the development of allergic bronchopulmonary aspergillosis in susceptible individuals. Aspergillus antigen consists of a number of chemically diverse components and their cumulative or synergistic effect may result in disease. When ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000065882
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:Flavonoids exert antiallergic and antioxidant effects. We investigated the efficacy of enzymatically modified isoquercitrin (EMIQ), a flavonoid, to relieve symptoms of pollinosis. METHODS:In a parallel-group, double-blind placebo-controlled study design, 20 subjects with Japanese cedar pollinosis took two c...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000205582
更新日期:2009-01-01 00:00:00
abstract::The JAK (Janus kinase) family of protein tyrosine kinases and the STATs (signal transducers and activators of transcription) have been shown to be activated in response to a number of cytokines and growth factors. In this study, we evaluated the activation of JAK/STAT pathway upon human interleukin-5 (hIL-5) stimulati...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237712
更新日期:1997-10-01 00:00:00
abstract:BACKGROUND:Sensitivity to the mite Blomia tropicalis is related to asthma in tropical climates, but correlates of sensitivity to B. tropicalis and its relationship to Dermatophagoides pteronyssinus sensitivity have not been widely examined in families with asthma. The main objective of this study was to determine preva...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000070927
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:Exhaled nitric oxide (NO) measurement at multiple exhalation flow rates allows calculation of flow-independent NO parameters: alveolar NO concentration, bronchial NO flux, bronchial diffusing capacity of NO and bronchial wall NO concentration. METHODS:In the present study, we measured the flow-independent N...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000155746
更新日期:2009-01-01 00:00:00
abstract::Recent data suggest that stem cell factor (SCF or c-kit ligand, KL) is a major regulator of human mast cells (MCs). In the present study, MCs derived from the lung (n = 8), uterus (n = 14) and heart (n = 4) were analyzed for expression of c-kit receptor and for responses to recombinant SCF. MCs of all organs tested we...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236568
更新日期:1993-01-01 00:00:00
abstract::This study concerned the way in which eosinophils adhere to their surroundings. Many fibrils were seen to adhere to the cell membrane of eosinophils migrating into the submucosa. Those eosinophils migrating into the epithelial layer adhered to the epithelial cells in 2 different ways: through the thin filaments bridgi...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237199
更新日期:1995-01-01 00:00:00
abstract::The modulatory role of hormones in the regulation of the immune response has been well documented. Here, we present some thoughts on how sex hormones affect immunity, particularly at mucosal sites. We further discuss the possible pitfalls and difficulties associated with analysis of the data. ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000236631
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Patients with allergic rhinoconjunctivitis are susceptible to both nasal and ocular symptoms. The conjunctival provocation test (CPT) is an established diagnostic procedure used in allergic rhinoconjunctivitis, particularly to document a patient's current reactivity to allergens. To date, there are no intern...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000355333
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Pseudomonas aeruginosa phospholipase C (PLC) is a critical component in the pathogenesis of severe P. aeruginosa infections. However, P. aeruginosa can produce a hemolytic (PLC-H) as well as a nonhemolytic (PLC-N) variant, both having a MW of about 77 kD. In the past, studies did not distinguish between both...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237441
更新日期:1997-02-01 00:00:00