Abstract:
:We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Hopper JT,Yu YT,Li D,Raymond A,Bostock M,Liko I,Mikhailov V,Laganowsky A,Benesch JL,Caffrey M,Nietlispach D,Robinson CVdoi
10.1038/nmeth.2691subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1206-8issue
12eissn
1548-7091issn
1548-7105pii
nmeth.2691journal_volume
10pub_type
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