Abstract：
:Quantitative measurements of cell-generated forces have heretofore required that cells be cultured on two-dimensional substrates. We describe a technique to quantitatively measure three-dimensional traction forces exerted by cells fully encapsulated in well-defined elastic hydrogel matrices. Using this approach we measured traction forces for several cell types in various contexts and revealed patterns of force generation attributable to morphologically distinct regions of cells as they extend into the surrounding matrix.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Legant WR,Miller JS,Blakely BL,Cohen DM,Genin GM,Chen CSdoi
10.1038/nmeth.1531subject
Has Abstractpub_date
2010-12-01 00:00:00pages
969-71issue
12eissn
1548-7091issn
1548-7105pii
nmeth.1531journal_volume
7pub_type
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