Abstract：
:Sequencing of RNA 3' ends has uncovered numerous sites that do not correspond to the termination sites of known transcripts. Through their 3' untranslated regions, protein-coding RNAs interact with RNA-binding proteins and microRNAs, which regulate many properties, including RNA stability and subcellular localization. We developed the terminal exon characterization (TEC) tool ( http://tectool.unibas.ch ), which can be used with RNA-sequencing data from any species for which a genome annotation that includes sites of RNA cleavage and polyadenylation is available. We discovered hundreds of previously unknown isoforms and cell-type-specific terminal exons in human cells. Ribosome profiling data revealed that many of these isoforms were translated. By applying TECtool to single-cell sequencing data, we found that the newly identified isoforms were expressed in subpopulations of cells. Thus, TECtool enables the identification of previously unknown isoforms in well-studied cell systems and in rare cell types.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Gruber AJ,Gypas F,Riba A,Schmidt R,Zavolan Mdoi
10.1038/s41592-018-0114-zsubject
Has Abstractpub_date
2018-10-01 00:00:00pages
832-836issue
10eissn
1548-7091issn
1548-7105pii
10.1038/s41592-018-0114-zjournal_volume
15pub_type
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