Abstract：
:Epistasis analysis, which reports on the extent to which the function of one gene depends on the presence of a second, is a powerful tool for studying the functional organization of the cell. Systematic genome-wide studies of epistasis, however, have been limited, with the majority of data being collected in the budding yeast, Saccharomyces cerevisiae. Here we present two 'pombe epistasis mapper' strategies, PEM-1 and PEM-2, which allow for high-throughput double mutant generation in the fission yeast, S. pombe. These approaches take advantage of a previously undescribed, recessive, cycloheximide-resistance mutation. Both systems can be used for genome-wide screens or for the generation of high-density, quantitative epistatic miniarray profiles (E-MAPs). Since S. cerevisiae and S. pombe are evolutionary distant, this methodology will provide insight into conserved biological pathways that are present in S. pombe, but not S. cerevisiae, and will enable a comprehensive analysis of the conservation of genetic interaction networks.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Roguev A,Wiren M,Weissman JS,Krogan NJdoi
10.1038/nmeth1098subject
Has Abstractpub_date
2007-10-01 00:00:00pages
861-6issue
10eissn
1548-7091issn
1548-7105pii
nmeth1098journal_volume
4pub_type
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