Abstract：
:We developed a highly sensitive parallel allele-specific sequencing (PASS) assay to simultaneously analyze a large number of viral genomes and detect minor drug-resistant populations at approximately 0.1-0.01% levels. Using this assay on samples from individuals infected with human immunodeficiency viruses (HIV), we successfully detected and quantified minor populations of drug-resistant viruses and performed linkage analysis of multiple-drug resistance mutations. This assay may serve as a useful tool to study drug resistance in HIV and other infectious agents.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Cai F,Chen H,Hicks CB,Bartlett JA,Zhu J,Gao Fdoi
10.1038/nmeth995subject
Has Abstractpub_date
2007-02-01 00:00:00pages
123-5issue
2eissn
1548-7091issn
1548-7105pii
nmeth995journal_volume
4pub_type
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