Abstract:
:Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in living cells. Here, we introduce a genetically encodable fluorescence resonance energy transfer (FRET) sensor for quantifying macromolecular crowding and discuss our application of the sensor in bacterial and mammalian cells.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Boersma AJ,Zuhorn IS,Poolman Bdoi
10.1038/nmeth.3257subject
Has Abstractpub_date
2015-03-01 00:00:00pages
227-9, 1 p following 229issue
3eissn
1548-7091issn
1548-7105pii
nmeth.3257journal_volume
12pub_type
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