Tracking transmitter-gated P2X cation channel activation in vitro and in vivo.

Abstract:

:We present a noninvasive approach to track activation of ATP-gated P2X receptors and potentially other transmitter-gated cation channels that show calcium fluxes. We genetically engineered rat P2X receptors to carry calcium sensors near the channel pore and tested this as a reporter for P2X(2) receptor opening. The method has several advantages over previous attempts to image P2X channel activation by fluorescence resonance energy transfer (FRET): notably, it reports channel opening rather than a conformation change in the receptor protein. Our FRET-based imaging approach can be used as a general method to track, in real time, the location, regional expression variation, mobility and activation of transmitter-gated P2X channels in living neurons in vitro and in vivo. This approach should help to determine when, where and how different receptors are activated during physiological processes.

journal_name

Nat Methods

journal_title

Nature methods

authors

Richler E,Chaumont S,Shigetomi E,Sagasti A,Khakh BS

doi

10.1038/nmeth1144

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

87-93

issue

1

eissn

1548-7091

issn

1548-7105

pii

nmeth1144

journal_volume

5

pub_type

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