Abstract：
:We describe a simple method for efficiently labeling cell-surface sialic acid-containing glycans on living animal cells. The method uses mild periodate oxidation to generate an aldehyde on sialic acids, followed by aniline-catalyzed oxime ligation with a suitable tag. Aniline catalysis dramatically accelerates oxime ligation, allowing use of low concentrations of aminooxy-biotin at neutral pH to label the majority of cell-surface sialylated glycoproteins while maintaining high cell viability.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Zeng Y,Ramya TN,Dirksen A,Dawson PE,Paulson JCdoi
10.1038/nmeth.1305subject
Has Abstractpub_date
2009-03-01 00:00:00pages
207-9issue
3eissn
1548-7091issn
1548-7105pii
nmeth.1305journal_volume
6pub_type
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