Abstract：
:Fluorescence microscopy is a powerful quantitative tool for exploring regulatory networks in single cells. However, the number of molecular species that can be measured simultaneously is limited by the spectral overlap between fluorophores. Here we demonstrate a simple but general strategy to drastically increase the capacity for multiplex detection of molecules in single cells by using optical super-resolution microscopy (SRM) and combinatorial labeling. As a proof of principle, we labeled mRNAs with unique combinations of fluorophores using fluorescence in situ hybridization (FISH), and resolved the sequences and combinations of fluorophores with SRM. We measured mRNA levels of 32 genes simultaneously in single Saccharomyces cerevisiae cells. These experiments demonstrate that combinatorial labeling and super-resolution imaging of single cells is a natural approach to bring systems biology into single cells.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Lubeck E,Cai Ldoi
10.1038/nmeth.2069subject
Has Abstractpub_date
2012-06-03 00:00:00pages
743-8issue
7eissn
1548-7091issn
1548-7105pii
nmeth.2069journal_volume
9pub_type
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