Abstract：
:We developed a quantitative PCR method featuring a reusable single-cell cDNA library immobilized on beads for measuring the expression of multiple genes in a single cell. We used this method to analyze multiple cDNA targets (from several copies to several hundred thousand copies) with an experimental error of 15.9% or less. This method is sufficiently accurate to investigate the heterogeneity of single cells.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Taniguchi K,Kajiyama T,Kambara Hdoi
10.1038/nmeth.1338subject
Has Abstractpub_date
2009-07-01 00:00:00pages
503-6issue
7eissn
1548-7091issn
1548-7105pii
nmeth.1338journal_volume
6pub_type
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