Abstract:
:We introduce a nonintrusive method exploiting single-cell variability after cell division to validate protein localization. We found that Clp proteases, widely reported to form biologically relevant foci, were uniformly distributed in Escherichia coli cells, and that many commonly used fluorescent proteins caused severe mislocalization when fused to homo-oligomers. Retagging five other reportedly foci-forming proteins with the most monomeric fluorescent protein tested suggests that the foci were caused by the fluorescent tags.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Landgraf D,Okumus B,Chien P,Baker TA,Paulsson Jdoi
10.1038/nmeth.1955subject
Has Abstractpub_date
2012-04-08 00:00:00pages
480-2issue
5eissn
1548-7091issn
1548-7105pii
nmeth.1955journal_volume
9pub_type
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