Abstract：
:We demonstrate gas cluster ion beam scanning electron microscopy (SEM), in which wide-area ion milling is performed on a series of thick tissue sections. This three-dimensional electron microscopy technique acquires datasets with <10 nm isotropic resolution of each section, and these can then be stitched together to span the sectioned volume. Incorporating gas cluster ion beam SEM into existing single-beam and multibeam SEM workflows should be straightforward, increasing reliability while improving z resolution by a factor of three or more.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Hayworth KJ,Peale D,Januszewski M,Knott GW,Lu Z,Xu CS,Hess HFdoi
10.1038/s41592-019-0641-2subject
Has Abstractpub_date
2020-01-01 00:00:00pages
68-71issue
1eissn
1548-7091issn
1548-7105pii
10.1038/s41592-019-0641-2journal_volume
17pub_type
杂志文章相关文献
NATURE METHODS文献大全abstract：:Focused-ion-beam scanning electron microscopy (FIB-SEM) has become an essential tool for studying neural tissue at resolutions below 10 nm × 10 nm × 10 nm, producing data sets optimized for automatic connectome tracing. We present a technical advance, ultrathick sectioning, which reliably subdivides embedded tissue sa...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.3292
更新日期：2015-04-01 00:00:00
abstract：:Cortical information processing relies on synaptic interactions between diverse classes of neurons with distinct electrophysiological and connection properties. Uncovering the operational principles of these elaborate circuits requires the probing of electrical activity from selected populations of defined neurons. He...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.1479
更新日期：2010-08-01 00:00:00
abstract：:Thermodynamic stability is fundamental to the biology of proteins. Information on protein stability is essential for studying protein structure and folding and can also be used indirectly to monitor protein-ligand or protein-protein interactions. While clearly valuable, the experimental determination of a protein's st...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth740
更新日期：2005-03-01 00:00:00
abstract：:The implementation of efficient technologies for the production of recombinant mammalian proteins remains an outstanding challenge in many structural and functional genomics programs. We have developed a new method for rapid identification of soluble protein expression in E. coli, based on a separation of soluble prot...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth767
更新日期：2005-07-01 00:00:00
abstract：:Brain atlases enable the mapping of labeled cells and projections from different brains onto a standard coordinate system. We address two issues in the construction and use of atlases. First, expert neuroanatomists ascertain the fine-scale pattern of brain tissue, the 'texture' formed by cellular organization, to defi...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-019-0328-8
更新日期：2019-04-01 00:00:00
abstract：:Protein complexes are key macromolecular machines of the cell, but their description remains incomplete. We and others previously reported an experimental strategy for global characterization of native protein assemblies based on chromatographic fractionation of biological extracts coupled to precision mass spectromet...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-019-0461-4
更新日期：2019-08-01 00:00:00
abstract：:High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamic...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.2964
更新日期：2014-07-01 00:00:00
abstract：: ...
journal_title：Nature methods
pub_type： 评论,杂志文章
doi：10.1038/s41592-018-0200-2
更新日期：2018-11-01 00:00:00
abstract：: ...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-018-0270-1
更新日期：2019-01-01 00:00:00
abstract：:Rational protein engineering requires a holistic understanding of protein function. Here, we apply deep learning to unlabeled amino-acid sequences to distill the fundamental features of a protein into a statistical representation that is semantically rich and structurally, evolutionarily and biophysically grounded. We...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-019-0598-1
更新日期：2019-12-01 00:00:00
abstract：:By combining activity-based proteomics and metabolomics, researchers have developed a new systems biology strategy for characterizing enzymes in the context of metabolic networks. ...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth0107-8b
更新日期：2007-01-01 00:00:00
abstract：:Physical modeling is increasingly important for generating insights into intracellular processes. We describe situations in which combined spatial and stochastic aspects of chemical reactions are needed to capture the relevant dynamics of biochemical systems. ...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.2253
更新日期：2012-12-01 00:00:00
abstract：:Electrically excitable cells are important in the normal functioning and in the pathophysiology of many biological processes. These cells are typically embedded in dense, heterogeneous tissues, rendering them difficult to target selectively with conventional electrical stimulation methods. The algal protein Channelrho...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth936
更新日期：2006-10-01 00:00:00
abstract：:Preprocessing of functional magnetic resonance imaging (fMRI) involves numerous steps to clean and standardize the data before statistical analysis. Generally, researchers create ad hoc preprocessing workflows for each dataset, building upon a large inventory of available tools. The complexity of these workflows has s...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-018-0235-4
更新日期：2019-01-01 00:00:00
abstract：:We describe a statistical analysis methodology designed to minimize the impact of off-target activities upon large-scale RNA interference (RNAi) screens in mammalian cells. Application of this approach enhances reconfirmation rates and facilitates the experimental validation of new gene activities through the probabil...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth1089
更新日期：2007-10-01 00:00:00
abstract：:In mass-spectrometry-based proteomics, the identification and quantification of peptides and proteins heavily rely on sequence database searching or spectral library matching. The lack of accurate predictive models for fragment ion intensities impairs the realization of the full potential of these approaches. Here, we...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-019-0426-7
更新日期：2019-06-01 00:00:00
abstract：:Researchers designed an enzyme to carry out the Diels-Alder reaction, an activity not found in nature. ...
journal_title：Nature methods
pub_type： 评论,杂志文章
doi：10.1038/nmeth0910-671
更新日期：2010-09-01 00:00:00
abstract：:We report an atmospheric pressure (AP) matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) setup with a lateral resolution of 1.4 μm, a mass resolution greater than 100,000, and accuracy below ±2 p.p.m. We achieved this by coupling a focusing objective with a numerical aperture (NA) of ...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.4071
更新日期：2017-01-01 00:00:00
abstract：:The importance of locating proteins in their context within cells has been heightened recently by the accomplishments in molecular structure and systems biology. Although light microscopy (LM) has been extensively used for mapping protein localization, many studies require the additional resolution of the electron mic...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth791
更新日期：2005-10-01 00:00:00
abstract：: ...
journal_title：Nature methods
pub_type： 评论,杂志文章
doi：10.1038/s41592-019-0343-9
更新日期：2019-03-01 00:00:00
abstract：:Numerous drugs and endogenous ligands bind to cell surface receptors leading to modulation of downstream signaling cascades and frequently to adaptation of the plasma membrane proteome. In-depth analysis of dynamic processes at the cell surface is challenging due to biochemical properties and low abundances of plasma ...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-020-01022-1
更新日期：2021-01-01 00:00:00
abstract：:We developed a highly sensitive parallel allele-specific sequencing (PASS) assay to simultaneously analyze a large number of viral genomes and detect minor drug-resistant populations at approximately 0.1-0.01% levels. Using this assay on samples from individuals infected with human immunodeficiency viruses (HIV), we s...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth995
更新日期：2007-02-01 00:00:00
abstract：:Circular RNAs (circRNAs) produced from back-spliced exons are widely expressed, but individual circRNA functions remain poorly understood owing to the lack of adequate methods for distinguishing circRNAs from cognate messenger RNAs with overlapping exons. Here, we report that CRISPR-RfxCas13d can effectively discrimin...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-020-01011-4
更新日期：2021-01-01 00:00:00
abstract：:CRISPR-based genetic screens are accelerating biological discovery, but current methods have inherent limitations. Widely used pooled screens are restricted to simple readouts including cell proliferation and sortable marker proteins. Arrayed screens allow for comprehensive molecular readouts such as transcriptome pro...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.4177
更新日期：2017-03-01 00:00:00
abstract：:We present single-cell interpretation via multikernel learning (SIMLR), an analytic framework and software which learns a similarity measure from single-cell RNA-seq data in order to perform dimension reduction, clustering and visualization. On seven published data sets, we benchmark SIMLR against state-of-the-art met...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.4207
更新日期：2017-04-01 00:00:00
abstract：:A quantitative high-throughput FRET-based method of screening fluorescent protein fusion libraries brings the promise of more detailed interactome maps. ...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth0207-112b
更新日期：2007-02-01 00:00:00
abstract：:Current extracellular multisite recordings suffer from low signal-to-noise ratio, limiting the monitoring to action potentials, and preclude detection of subthreshold synaptic potentials. Here we report an approach to induce Aplysia californica neurons to actively engulf protruding microelectrodes, providing 'in-cell ...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.1420
更新日期：2010-03-01 00:00:00
abstract：:An uncharged CoA precursor that can enter the cell is used for covalent, site-specific labeling of proteins inside living cells. ...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth1106-872b
更新日期：2006-11-01 00:00:00
abstract：:Methods for visualizing protein or nucleic acid motifs have traditionally relied upon residue frequencies to graphically scale character heights. We describe the pLogo, a motif visualization in which residue heights are scaled relative to their statistical significance. A pLogo generation tool is publicly available at...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.2646
更新日期：2013-12-01 00:00:00
abstract：:Dissecting the relationship between genotype and phenotype is one of the central goals in developmental biology and medicine. Transcriptome analysis is a powerful strategy to connect genotype to phenotype of a cell. Here we review the history, progress, potential applications and future developments of single-cell tra...
journal_title：Nature methods
pub_type： 历史文章,杂志文章,评审
doi：10.1038/nmeth.1557
更新日期：2011-04-01 00:00:00
