Abstract:
:Current extracellular multisite recordings suffer from low signal-to-noise ratio, limiting the monitoring to action potentials, and preclude detection of subthreshold synaptic potentials. Here we report an approach to induce Aplysia californica neurons to actively engulf protruding microelectrodes, providing 'in-cell recordings' of subthreshold synaptic and action potentials with signal-to-noise ratio that matches that of conventional intracellular recordings. Implementation of this approach may open new vistas in neuroscience and biomedical applications.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Hai A,Shappir J,Spira MEdoi
10.1038/nmeth.1420subject
Has Abstractpub_date
2010-03-01 00:00:00pages
200-2issue
3eissn
1548-7091issn
1548-7105pii
nmeth.1420journal_volume
7pub_type
杂志文章相关文献
NATURE METHODS文献大全abstract::Despite widespread use of CRISPR, comprehensive data on the frequency and impact of Cas9-mediated off-targets in modified rodents are limited. Here we present deep-sequencing data from 81 genome-editing projects on mouse and rat genomes at 1,423 predicted off-target sites, 32 of which were confirmed, and show that hig...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0011-5
更新日期:2018-07-01 00:00:00
abstract::Single-cell transcriptomics provides an opportunity to characterize cell-type-specific transcriptional networks, intercellular signaling pathways and cellular diversity with unprecedented resolution by profiling thousands of cells in a single experiment. However, owing to the unique statistical properties of scRNA-seq...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-019-0372-4
更新日期:2019-05-01 00:00:00
abstract::Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luci...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.2839
更新日期:2014-04-01 00:00:00
abstract::Methods for genomic analysis at single-cell resolution enable new understanding of complex biological phenomena. Single-cell techniques, ranging from flow cytometry and microfluidics to PCR and sequencing, are used to understand the cellular composition of complex tissues, find new microbial species and perform genome...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth0411-311
更新日期:2011-04-01 00:00:00
abstract::Cryogenic electron microscopy (cryo-EM) is widely used to study biological macromolecules that comprise regions with disorder, flexibility or partial occupancy. For example, membrane proteins are often kept in solution with detergent micelles and lipid nanodiscs that are locally disordered. Such spatial variability ne...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-020-00990-8
更新日期:2020-12-01 00:00:00
abstract::Glycosylation, which represents the most complex posttranslational modification (PTM) event during protein maturation, has a vital role in biological processes. Glycan biosynthesis is orchestrated by numerous glycosyltransferases, each displaying different selectivities for multiple reaction sites. The precise specifi...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth1050
更新日期:2007-07-01 00:00:00
abstract::Despite the vital role of mechanical forces in biology, it still remains a challenge to image cellular force with sub-100-nm resolution. Here, we present tension points accumulation for imaging in nanoscale topography (tPAINT), integrating molecular tension probes with the DNA points accumulation for imaging in nanosc...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-020-0929-2
更新日期:2020-10-01 00:00:00
abstract::Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sam...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1871
更新日期:2012-02-05 00:00:00
abstract:: ...
journal_title:Nature methods
pub_type: 评论,杂志文章
doi:10.1038/s41592-019-0315-0
更新日期:2019-02-01 00:00:00
abstract::We developed a quantitative PCR method featuring a reusable single-cell cDNA library immobilized on beads for measuring the expression of multiple genes in a single cell. We used this method to analyze multiple cDNA targets (from several copies to several hundred thousand copies) with an experimental error of 15.9% or...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1338
更新日期:2009-07-01 00:00:00
abstract::We describe a protein quantification method called neutron encoding that exploits the subtle mass differences caused by nuclear binding energy variation in stable isotopes. These mass differences are synthetically encoded into amino acids and incorporated into yeast and mouse proteins via metabolic labeling. Mass spec...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.2378
更新日期:2013-04-01 00:00:00
abstract::We discuss the advantages and challenges of the open-source strategy in biological image analysis and argue that its full impact will not be realized without better support and recognition of software engineers' contributions to the biological sciences and more support of this development model from funders and instit...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.2082
更新日期:2012-06-28 00:00:00
abstract::The successful in vitro evolution of a DNA enzyme based on a ribozyme could offer insights into early evolutionary events and provide guidelines for designing hardier catalytic oligonucleotides. ...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth0606-424b
更新日期:2006-06-01 00:00:00
abstract::We describe factored spectrally transformed linear mixed models (FaST-LMM), an algorithm for genome-wide association studies (GWAS) that scales linearly with cohort size in both run time and memory use. On Wellcome Trust data for 15,000 individuals, FaST-LMM ran an order of magnitude faster than current efficient algo...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1681
更新日期:2011-09-04 00:00:00
abstract::The reconstruction of neuronal populations, a key step in understanding neural circuits, remains a challenge in the presence of densely packed neurites. Here we achieved automatic reconstruction of neuronal populations by partially mimicking human strategies to separate individual neurons. For populations not resolvab...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3662
更新日期:2016-01-01 00:00:00
abstract::Human cortical organoids (hCOs), derived from human embryonic stem cells (hESCs), provide a platform to study human brain development and diseases in complex three-dimensional tissue. However, current hCOs lack microvasculature, resulting in limited oxygen and nutrient delivery to the inner-most parts of hCOs. We engi...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-019-0586-5
更新日期:2019-11-01 00:00:00
abstract::Optimism about biomedicine is challenged by the increasingly complex ethical, legal and social issues it raises. Reporting of scientific methods is no longer sufficient to address the complex relationship between science and society. To promote 'ethical reproducibility', we call for transparent reporting of research e...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.2564
更新日期:2013-09-01 00:00:00
abstract::We developed a multiplex sequencing-by-synthesis method combining terminal phosphate-labeled fluorogenic nucleotides (TPLFNs) and resealable polydimethylsiloxane (PDMS) microreactors. In the presence of phosphatase, primer extension by DNA polymerase using nonfluorescent TPLFNs generates fluorophores, which are confin...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1629
更新日期:2011-06-12 00:00:00
abstract::Identifying groups of similar objects is a popular first step in biomedical data analysis, but it is error-prone and impossible to perform manually. Many computational methods have been developed to tackle this problem. Here we assessed 13 well-known methods using 24 data sets ranging from gene expression to protein d...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3583
更新日期:2015-11-01 00:00:00
abstract::Quantification of cell-cycle state at a single-cell level is essential to understand fundamental three-dimensional (3D) biological processes such as tissue development and cancer. Analysis of 3D in vivo images, however, is very challenging. Today's best practice, manual annotation of select image events, generates arb...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3363
更新日期:2015-06-01 00:00:00
abstract::Single-cell RNA sequencing has enabled the decomposition of complex tissues into functionally distinct cell types. Often, investigators wish to assign cells to cell types through unsupervised clustering followed by manual annotation or via 'mapping' to existing data. However, manual interpretation scales poorly to lar...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-019-0529-1
更新日期:2019-10-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature methods
pub_type: 已发布勘误
doi:10.1038/s41592-019-0660-z
更新日期:2019-12-01 00:00:00
abstract::We have developed a versatile, potent technique for imaging cells in culture and in vivo by expressing a metabolically biotinylated cell-surface receptor and visualizing it with labeled streptavidin moieties. The recombinant reporter protein, which incorporates a biotin acceptor peptide (BAP) between an N-terminal sig...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth875
更新日期:2006-05-01 00:00:00
abstract::We describe Trans-ABySS, a de novo short-read transcriptome assembly and analysis pipeline that addresses variation in local read densities by assembling read substrings with varying stringencies and then merging the resulting contigs before analysis. Analyzing 7.4 gigabases of 50-base-pair paired-end Illumina reads f...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1517
更新日期:2010-11-01 00:00:00
abstract::We present functional ultrasound (fUS), a method for imaging transient changes in blood volume in the whole brain at better spatiotemporal resolution than with other functional brain imaging modalities. fUS uses plane-wave illumination at high frame rate and can measure blood volumes in smaller vessels than previous u...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1641
更新日期:2011-07-03 00:00:00
abstract::We demonstrate tracking of protein structural changes with time-resolved wide-angle X-ray scattering (TR-WAXS) with nanosecond time resolution. We investigated the tertiary and quaternary conformational changes of human hemoglobin under nearly physiological conditions triggered by laser-induced ligand photolysis. We a...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1255
更新日期:2008-10-01 00:00:00
abstract::Cryogenic electron microscopy (cryo-EM) maps are now at the point where resolvability of individual atoms can be achieved. However, resolvability is not necessarily uniform throughout the map. We introduce a quantitative parameter to characterize the resolvability of individual atoms in cryo-EM maps, the map Q-score. ...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-020-0731-1
更新日期:2020-03-01 00:00:00
abstract::Sequencing of RNA 3' ends has uncovered numerous sites that do not correspond to the termination sites of known transcripts. Through their 3' untranslated regions, protein-coding RNAs interact with RNA-binding proteins and microRNAs, which regulate many properties, including RNA stability and subcellular localization....
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0114-z
更新日期:2018-10-01 00:00:00
abstract::Circuit mapping requires knowledge of both structural and functional connectivity between cells. Although optical tools have been made to assess either the morphology and projections of neurons or their activity and functional connections, few probes integrate this information. We have generated a family of photoactiv...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3480
更新日期:2015-09-01 00:00:00
abstract::Single-molecule localization microscopy techniques have proven to be essential tools for quantitatively monitoring biological processes at unprecedented spatial resolution. However, these techniques are very low throughput and are not yet compatible with fully automated, multiparametric cellular assays. This shortcomi...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4486
更新日期:2017-12-01 00:00:00