Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy.

Abstract:

:High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.

journal_name

Nat Methods

journal_title

Nature methods

authors

Prevedel R,Yoon YG,Hoffmann M,Pak N,Wetzstein G,Kato S,Schrödel T,Raskar R,Zimmer M,Boyden ES,Vaziri A

doi

10.1038/nmeth.2964

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

727-730

issue

7

eissn

1548-7091

issn

1548-7105

journal_volume

11

pub_type

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