Abstract：
:Focused-ion-beam scanning electron microscopy (FIB-SEM) has become an essential tool for studying neural tissue at resolutions below 10 nm × 10 nm × 10 nm, producing data sets optimized for automatic connectome tracing. We present a technical advance, ultrathick sectioning, which reliably subdivides embedded tissue samples into chunks (20 μm thick) optimally sized and mounted for efficient, parallel FIB-SEM imaging. These chunks are imaged separately and then 'volume stitched' back together, producing a final three-dimensional data set suitable for connectome tracing.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Hayworth KJ,Xu CS,Lu Z,Knott GW,Fetter RD,Tapia JC,Lichtman JW,Hess HFdoi
10.1038/nmeth.3292subject
Has Abstractpub_date
2015-04-01 00:00:00pages
319-22issue
4eissn
1548-7091issn
1548-7105pii
nmeth.3292journal_volume
12pub_type
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