Abstract：
:Ribonucleoproteins (RNPs) are key regulators of cellular function. We established an efficient approach, crosslinking of segmentally isotope-labeled RNA and tandem mass spectrometry (CLIR-MS/MS), to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. The approach was tested on polypyrimidine tract binding protein 1 and U1 small nuclear RNP. Our method provides distance restraints to support integrative atomic-scale structural modeling and to gain mechanistic insights into RNP-regulated processes.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Dorn G,Leitner A,Boudet J,Campagne S,von Schroetter C,Moursy A,Aebersold R,Allain FHdoi
10.1038/nmeth.4235subject
Has Abstractpub_date
2017-05-01 00:00:00pages
487-490issue
5eissn
1548-7091issn
1548-7105journal_volume
14pub_type
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