Abstract:
:Despite its biological importance, tRNA has not been adequately sequenced by standard methods because of its abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieved efficient and quantitative tRNA sequencing in HEK293T cells by using engineered demethylases to remove base methylations and a highly processive thermostable group II intron reverse transcriptase to overcome these obstacles. Our method, DM-tRNA-seq, should be applicable to investigations of tRNA in all organisms.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Zheng G,Qin Y,Clark WC,Dai Q,Yi C,He C,Lambowitz AM,Pan Tdoi
10.1038/nmeth.3478subject
Has Abstractpub_date
2015-09-01 00:00:00pages
835-837issue
9eissn
1548-7091issn
1548-7105journal_volume
12pub_type
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