Abstract：
:Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its homologs in protein labeling. Drawing on structural analysis of the dimer interface, we identified a bacteriophytochrome in the sequence database that is monomeric in truncated form and engineered it into a naturally monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live cells, Drosophila and zebrafish. It should be useful in molecular, cell and developmental biology.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Yu D,Baird MA,Allen JR,Howe ES,Klassen MP,Reade A,Makhijani K,Song Y,Liu S,Murthy Z,Zhang SQ,Weiner OD,Kornberg TB,Jan YN,Davidson MW,Shu Xdoi
10.1038/nmeth.3447subject
Has Abstractpub_date
2015-08-01 00:00:00pages
763-5issue
8eissn
1548-7091issn
1548-7105pii
nmeth.3447journal_volume
12pub_type
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