Abstract:
:We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington's disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Ramdzan YM,Polling S,Chia CP,Ng IH,Ormsby AR,Croft NP,Purcell AW,Bogoyevitch MA,Ng DC,Gleeson PA,Hatters DMdoi
10.1038/nmeth.1930subject
Has Abstractpub_date
2012-03-18 00:00:00pages
467-70issue
5eissn
1548-7091issn
1548-7105pii
nmeth.1930journal_volume
9pub_type
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