Tracking protein aggregation and mislocalization in cells with flow cytometry.

Abstract:

:We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington's disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.

journal_name

Nat Methods

journal_title

Nature methods

authors

Ramdzan YM,Polling S,Chia CP,Ng IH,Ormsby AR,Croft NP,Purcell AW,Bogoyevitch MA,Ng DC,Gleeson PA,Hatters DM

doi

10.1038/nmeth.1930

subject

Has Abstract

pub_date

2012-03-18 00:00:00

pages

467-70

issue

5

eissn

1548-7091

issn

1548-7105

pii

nmeth.1930

journal_volume

9

pub_type

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