Abstract：
:The implementation of efficient technologies for the production of recombinant mammalian proteins remains an outstanding challenge in many structural and functional genomics programs. We have developed a new method for rapid identification of soluble protein expression in E. coli, based on a separation of soluble protein from inclusion bodies by a filtration step at the colony level. The colony filtration (CoFi) blot is very well suited to screen libraries, and in the present work we used it to screen a deletion mutagenesis library.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Cornvik T,Dahlroth SL,Magnusdottir A,Herman MD,Knaust R,Ekberg M,Nordlund Pdoi
10.1038/nmeth767keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
507-9issue
7eissn
1548-7091issn
1548-7105pii
nmeth767journal_volume
2pub_type
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