Abstract:
:To enable the detection of expression signatures specific to individual cells, we developed PLAYR (proximity ligation assay for RNA), a method for highly multiplexed transcript quantification by flow and mass cytometry that is compatible with standard antibody staining. When used with mass cytometry, PLAYR allowed for the simultaneous quantification of more than 40 different mRNAs and proteins. In primary cells, we quantified multiple transcripts, with the identity and functional state of each analyzed cell defined on the basis of the expression of a separate set of transcripts or proteins. By expanding high-throughput deep phenotyping of cells beyond protein epitopes to include RNA expression, PLAYR opens a new avenue for the characterization of cellular metabolism.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Frei AP,Bava FA,Zunder ER,Hsieh EW,Chen SY,Nolan GP,Gherardini PFdoi
10.1038/nmeth.3742subject
Has Abstractpub_date
2016-03-01 00:00:00pages
269-75issue
3eissn
1548-7091issn
1548-7105pii
nmeth.3742journal_volume
13pub_type
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