Abstract:
:RNA-guided Cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have dramatically transformed our ability to edit the genomes of diverse organisms. We believe tools and techniques based on Cas9, a single unifying factor capable of colocalizing RNA, DNA and protein, will grant unprecedented control over cellular organization, regulation and behavior. Here we describe the Cas9 targeting methodology, detail current and prospective engineering advances and suggest potential applications ranging from basic science to the clinic.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Mali P,Esvelt KM,Church GMdoi
10.1038/nmeth.2649subject
Has Abstractpub_date
2013-10-01 00:00:00pages
957-63issue
10eissn
1548-7091issn
1548-7105pii
nmeth.2649journal_volume
10pub_type
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