Abstract:
:Membrane proteins are largely underrepresented among available atomic-resolution structures. The use of detergents in protein purification procedures hinders the formation of well-ordered crystals for X-ray crystallography and leads to slower molecular tumbling, impeding the application of solution-state NMR. Solid-state magic-angle spinning NMR spectroscopy is an emerging method for membrane-protein structural biology that can overcome these technical problems. Here we present the solid-state NMR structure of the transmembrane domain of the Yersinia enterocolitica adhesin A (YadA). The sample was derived from crystallization trials that yielded only poorly diffracting microcrystals. We solved the structure using a single, uniformly (13)C- and (15)N-labeled sample. In addition, solid-state NMR allowed us to acquire information on the flexibility and mobility of parts of the structure, which, in combination with evolutionary conservation information, presents new insights into the autotransport mechanism of YadA.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Shahid SA,Bardiaux B,Franks WT,Krabben L,Habeck M,van Rossum BJ,Linke Ddoi
10.1038/nmeth.2248subject
Has Abstractpub_date
2012-12-01 00:00:00pages
1212-7issue
12eissn
1548-7091issn
1548-7105pii
nmeth.2248journal_volume
9pub_type
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