Abstract:
:Caenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin that allows the rapid and easy isolation of large populations of transgenic worms. This approach is sufficiently powerful to select single-copy transgenes, does not require any particular genetic background and also works in C. briggsae.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Semple JI,Garcia-Verdugo R,Lehner Bdoi
10.1038/nmeth.1495subject
Has Abstractpub_date
2010-09-01 00:00:00pages
725-7issue
9eissn
1548-7091issn
1548-7105pii
nmeth.1495journal_volume
7pub_type
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