Abstract：
:RNAs are ideal for the design of gene switches that can monitor and program cellular behavior because of their high modularity and predictable structure-function relationship. We have assembled an expression platform with an embedded modular ribozyme scaffold that correlates self-cleavage activity of designer ribozymes with transgene translation in bacteria and mammalian cells. A design approach devised to screen ribozyme libraries in bacteria and validate variants with functional tertiary stem-loop structures in mammalian cells resulted in a designer ribozyme with a protein-binding nutR-boxB stem II and a selected matching stem I. In a mammalian expression context, this designer ribozyme exhibited dose-dependent translation control by the N-peptide, had rapid induction kinetics and could be combined with classic small molecule-responsive transcription control modalities to construct complex, programmable genetic circuits.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Ausländer S,Stücheli P,Rehm C,Ausländer D,Hartig JS,Fussenegger Mdoi
10.1038/nmeth.3136subject
Has Abstractpub_date
2014-11-01 00:00:00pages
1154-60issue
11eissn
1548-7091issn
1548-7105pii
nmeth.3136journal_volume
11pub_type
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