Nm-seq maps 2'-O-methylation sites in human mRNA with base precision.

Abstract:

:The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.

journal_name

Nat Methods

journal_title

Nature methods

authors

Dai Q,Moshitch-Moshkovitz S,Han D,Kol N,Amariglio N,Rechavi G,Dominissini D,He C

doi

10.1038/nmeth.4294

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

695-698

issue

7

eissn

1548-7091

issn

1548-7105

pii

nmeth.4294

journal_volume

14

pub_type

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