Abstract:
:The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Dai Q,Moshitch-Moshkovitz S,Han D,Kol N,Amariglio N,Rechavi G,Dominissini D,He Cdoi
10.1038/nmeth.4294subject
Has Abstractpub_date
2017-07-01 00:00:00pages
695-698issue
7eissn
1548-7091issn
1548-7105pii
nmeth.4294journal_volume
14pub_type
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