Abstract：
:High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal stratum pyramidale at ∼1-mm depth within an intact mouse brain. Our method creates opportunities for noninvasive recording of neuronal activity with high spatial and temporal resolution deep within scattering brain tissues.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Ouzounov DG,Wang T,Wang M,Feng DD,Horton NG,Cruz-Hernández JC,Cheng YT,Reimer J,Tolias AS,Nishimura N,Xu Cdoi
10.1038/nmeth.4183subject
Has Abstractpub_date
2017-04-01 00:00:00pages
388-390issue
4eissn
1548-7091issn
1548-7105pii
nmeth.4183journal_volume
14pub_type
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