Abstract：
:We report a simple and generic method for the direct transfer of protein complexes separated by native gel electrophoresis to electron microscopy grids. After transfer, sufficient material remains in the gel for identification and characterization by mass spectrometry. The method should facilitate higher-throughput single-particle analysis by substantially reducing the time needed for protein purification, as demonstrated for three complexes from Thermoplasma acidophilum.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Knispel RW,Kofler C,Boicu M,Baumeister W,Nickell Sdoi
10.1038/nmeth.1840subject
Has Abstractpub_date
2012-01-08 00:00:00pages
182-4issue
2eissn
1548-7091issn
1548-7105pii
nmeth.1840journal_volume
9pub_type
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