Abstract:
:We have developed a single-plasmid system for the efficient bacterial expression of mutant proteins containing unnatural amino acids at specific sites designated by amber nonsense codons. In this system, multiple copies of a gene encoding an amber suppressor tRNA derived from a Methanocaldococcus jannaschii tyrosyl-tRNA (MjtRNATyrCUA) are expressed under control of the proK promoter and terminator, and a gene encoding the desired mutant M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS) is expressed under control of a mutant glnS (glnS') promoter.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Ryu Y,Schultz PGdoi
10.1038/nmeth864keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
263-5issue
4eissn
1548-7091issn
1548-7105pii
nmeth864journal_volume
3pub_type
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