Abstract：
:Cortical information processing relies on synaptic interactions between diverse classes of neurons with distinct electrophysiological and connection properties. Uncovering the operational principles of these elaborate circuits requires the probing of electrical activity from selected populations of defined neurons. Here we show that genetically encoded voltage-sensitive fluorescent proteins (VSFPs) provide an optical voltage report from targeted neurons in culture, acute brain slices and living mice. By expressing VSFPs in pyramidal cells of mouse somatosensory cortex, we also demonstrate that these probes can report cortical electrical responses to single sensory stimuli in vivo. These protein-based voltage probes will facilitate the analysis of cortical circuits in genetically defined cell populations and are hence a valuable addition to the optogenetic toolbox.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Akemann W,Mutoh H,Perron A,Rossier J,Knöpfel Tdoi
10.1038/nmeth.1479subject
Has Abstractpub_date
2010-08-01 00:00:00pages
643-9issue
8eissn
1548-7091issn
1548-7105pii
nmeth.1479journal_volume
7pub_type
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