Abstract：
:Counting molecules in complexes is challenging, even with super-resolution microscopy. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative points accumulation in nanoscale topography (qPAINT), works independently of dye photophysics for robust counting with high precision and accuracy over a wide dynamic range. qPAINT was benchmarked on DNA nanostructures and demonstrated for cellular applications by quantifying proteins in situ and the number of single-molecule FISH probes bound to an mRNA target.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Jungmann R,Avendaño MS,Dai M,Woehrstein JB,Agasti SS,Feiger Z,Rodal A,Yin Pdoi
10.1038/nmeth.3804subject
Has Abstractpub_date
2016-05-01 00:00:00pages
439-42issue
5eissn
1548-7091issn
1548-7105pii
nmeth.3804journal_volume
13pub_type
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