Abstract：
:We examined cell cycle-dependent changes in the proteome of human cells by systematically measuring protein dynamics in individual living cells. We used time-lapse microscopy to measure the dynamics of a random subset of 20 nuclear proteins, each tagged with yellow fluorescent protein (YFP) at its endogenous chromosomal location. We synchronized the cells in silico by aligning protein dynamics in each cell between consecutive divisions. We observed widespread (40%) cell-cycle dependence of nuclear protein levels and detected previously unknown cell cycle-dependent localization changes. This approach to dynamic proteomics can aid in discovery and accurate quantification of the extensive regulation of protein concentration and localization in individual living cells.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Sigal A,Milo R,Cohen A,Geva-Zatorsky N,Klein Y,Alaluf I,Swerdlin N,Perzov N,Danon T,Liron Y,Raveh T,Carpenter AE,Lahav G,Alon Udoi
10.1038/nmeth892subject
Has Abstractpub_date
2006-07-01 00:00:00pages
525-31issue
7eissn
1548-7091issn
1548-7105pii
nmeth892journal_volume
3pub_type
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