Abstract:
:Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Evans MS,Chaurette JP,Adams ST Jr,Reddy GR,Paley MA,Aronin N,Prescher JA,Miller SCdoi
10.1038/nmeth.2839subject
Has Abstractpub_date
2014-04-01 00:00:00pages
393-5issue
4eissn
1548-7091issn
1548-7105pii
nmeth.2839journal_volume
11pub_type
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