Abstract:
:The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Biteen JS,Thompson MA,Tselentis NK,Bowman GR,Shapiro L,Moerner WEdoi
10.1038/nmeth.1258subject
Has Abstractpub_date
2008-11-01 00:00:00pages
947-9issue
11eissn
1548-7091issn
1548-7105pii
nmeth.1258journal_volume
5pub_type
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