Abstract：
:We describe a protein quantification method called neutron encoding that exploits the subtle mass differences caused by nuclear binding energy variation in stable isotopes. These mass differences are synthetically encoded into amino acids and incorporated into yeast and mouse proteins via metabolic labeling. Mass spectrometry analysis with high mass resolution (>200,000) reveals the isotopologue-embedded peptide signals, permitting quantification. Neutron encoding will enable highly multiplexed proteome analysis with excellent dynamic range and accuracy.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Hebert AS,Merrill AE,Bailey DJ,Still AJ,Westphall MS,Strieter ER,Pagliarini DJ,Coon JJdoi
10.1038/nmeth.2378subject
Has Abstractpub_date
2013-04-01 00:00:00pages
332-4issue
4eissn
1548-7091issn
1548-7105pii
nmeth.2378journal_volume
10pub_type
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