Abstract:
:The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Toettcher JE,Gong D,Lim WA,Weiner ODdoi
10.1038/nmeth.1700subject
Has Abstractpub_date
2011-09-11 00:00:00pages
837-9issue
10eissn
1548-7091issn
1548-7105pii
nmeth.1700journal_volume
8pub_type
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