Abstract:
:A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Bajar BT,Lam AJ,Badiee RK,Oh YH,Chu J,Zhou XX,Kim N,Kim BB,Chung M,Yablonovitch AL,Cruz BF,Kulalert K,Tao JJ,Meyer T,Su XD,Lin MZdoi
10.1038/nmeth.4045subject
Has Abstractpub_date
2016-12-01 00:00:00pages
993-996issue
12eissn
1548-7091issn
1548-7105pii
nmeth.4045journal_volume
13pub_type
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